IFN-β inhibits proliferation and promotes apoptosis of AML cells by STAT1-induced SARI
10.3969/j.issn.1000-484X.2024.06.004
- VernacularTitle:IFN-β通过STAT1诱导SARI表达抑制AML细胞增殖并促进凋亡
- Author:
Yanfeng LIN
1
;
Xiaoying HONG
;
Yingying HUANG
;
Xiaohua WANG
;
Wei WU
;
Donghong LIN
;
Yan XUE
Author Information
1. 福建医科大学医学技术与工程学院,福州 350004
- Keywords:
IFN-β;
SARI;
STAT1;
AML;
Proliferation;
Apoptosis
- From:
Chinese Journal of Immunology
2024;40(6):1137-1141
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effect of SARI expression induced by IFN-β on proliferation and apoptosis of acute myelo-blastic leukemia(AML)cells,and to explore its potential regulatory molecules.Methods:qPCR and Western blot were used to screen AML cells with low SARI expression as experimental cell lines.AML cells were treated with different concentrations of IFN-β,and expression of SARI was detected by qPCR and Western blot at different time to select appropriate concentration and time of IFN-β.RNA-Seq transcriptome sequencing and KEGG enrichment analysis were used to preliminarily screen potential regulatory molecules of IFN-β-induced SARI expression in AML cells.AML cells were treated with corresponding molecular inhibitors combined with IFN-β,cell proliferation was detected by MTS assay,and apoptosis was detected by flow cytometry.To clear this molecule was involved in IFN-β-induced SARI expression on AML cell proliferation and apoptosis.Results:SARI expression in HL60 and NB4 cells were rela-tively decreased,so they were selected as experimental cell lines.After treatment with 1 ng/ml IFN-β for 12 h,SARI expression in AML cells was increased,cell proliferation was inhibited and apoptosis were increased.STAT1 was screened as a potential regulatory mole-cule for IFN-β-induced SARI expression.After inhibiting STAT1,effects of IFN-β on SARI expression,proliferation inhibition and apop-tosis promotion of AML cells were reversed significantly.Conclusion:IFN-β can promote SARI expression in AML cells by STAT1,in-hibit cell proliferation and promote apoptosis.
