Study on the mechanism of Buyang Huanwu decoction in inhibiting atherosclerotic le-sion formation and reducing serum inflammatory factors in ApoE-/-mice
10.20039/j.cnki.1007-3949.2024.04.002
- VernacularTitle:补阳还五汤加减抑制ApoE-/-小鼠动脉粥样硬化病变形成及降低血清炎症因子水平的机制
- Author:
Zengguang FAN
1
;
Ye YUAN
Author Information
1. 江西中医药大学附属医院,江西省南昌市 330006
- Keywords:
atherosclerosis;
Buyang Huanwu decoction modified;
phenotypic transformation of vascular smooth muscle cells;
inflammatory response;
Wnt/β-catenin signaling pathway
- From:
Chinese Journal of Arteriosclerosis
2024;32(4):285-292
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of Buyang Huanwu decoction on anti-atherosclerosis by regulating the phe-notypic transformation of vascular smooth muscle cells and serum inflammatory response based on Wnt/β-catenin signaling pathway.Methods 50 ApoE-/-mice were randomly divided into model group,Buyang Huanwu decoction modified(low,medium and high dose)groups,and atorvastatin group,with 10 mice in each group;10 C57BL/6J mice with the same genetic background were set as the control group.The mice of control group were fed with regular forages,while the mice of other five groups were fed with high-fat forages for 8 weeks for model replication.From the 9th week,they were continuously gavaged for 4 weeks.After 12 weeks,the mouse aortic roots were isolated and paraffin sections were pre-pared.Oil red O staining was used to observe the lipid content of aortic sinus.Immunohistochemical method was used to detect the expression level of bone bridge protein(OPN).Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-ot),interleukin-1β(IL-1β)and monocyte che-moattractant protein-1(MCP-1)in serum.Western blot was used to detect the protein expressions of Wnt1,β-catenin and c-myc in mice aorta.RT-PCR was used to detect the expressions of Wnt1 and β-catenin gene.Results Buyang Huanwu decoction modified(low,medium and high dose)could reduce the lipid content and OPN expression in the aortic sinus of As model mice,reduce serum levels of IL-6 and TNF-α,IL-1β and MCP-1 in mice,and downregulate the expres-sion of Wnt1 and β-catenin protein and gene.Conclusion Buyang Huanwu decoction modified exerts an anti-athero-sclerosis effect by regulating inflammatory response,and some of the mechanisms may be related to inhibiting the activation of the Wnt/β-catenin signaling pathway.
