Role of reactive oxygen species/silent information regulator 1 in hyperoxia-induced bronchial epithelial cell injury
10.7499/j.issn.1008-8830.2404120
- VernacularTitle:活性氧簇/沉默信息调节因子1在高氧致支气管上皮细胞损伤中的作用
- Author:
Kun YANG
1
;
Yue WU
;
Rong ZHANG
;
Xiao-Ping LEI
;
Lan KANG
;
Wen-Bin DONG
Author Information
1. 西南医科大学附属医院儿童医学中心新生儿科/四川省出生缺陷临床医学研究中心,四川泸州 646000
- Keywords:
Mitochondrial injury;
Hyperoxia;
Reactive oxygen species;
Silent information regulator 1;
Human bronchial epithelial cell
- From:
Chinese Journal of Contemporary Pediatrics
2024;26(8):852-860
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of reactive oxygen species(ROS)/silent information regulator 1(SIRT1)on hyperoxia-induced mitochondrial injury in BEAS-2B cells.Methods The experiment was divided into three parts.In the first part,cells were divided into H0,H6,H12,H24,and H48 groups.In the second part,cells were divided into control group,H48 group,H48 hyperoxia+SIRT1 inhibitor group(H48+EX 527 group),and H48 hyperoxia+SIRT1 agonist group(H48+SRT1720 group).In the third part,cells were divided into control group,48-hour hyperoxia+N-acetylcysteine group(H48+NAC group),and H48 group.The ROS kit was used to measure the level of ROS.Western blot and immunofluorescent staining were used to measure the expression levels of SIRT1 and mitochondria-related proteins.Transmission electron microscopy was used to observe the morphology of mitochondria.Results Compared with the H0 group,the H6,H12,H24,and H48 groups had a significantly increased fluorescence intensity of ROS(P<0.05),the H48 group had significant reductions in the expression levels of SIRT1 protein and mitochondria-related proteins(P<0.05),and the H24 and H48 groups had a significant reduction in the fluorescence intensity of mitochondria-related proteins(P<0.05).Compared with the H48 group,the H48+SRT1720 group had significant increases in the expression levels of mitochondria-related proteins and the mitochondrial aspect ratio(P<0.05),and the H48+EX 527 group had a significant reduction in the mitochondrial area(P<0.05).Compared with the H48 group,the H48+NAC group had a significantly decreased fluorescence intensity of ROS(P<0.05)and significantly increased levels of SIRT1 protein,mitochondria-related proteins,mitochondrial area,and mitochondrial aspect ratio(P<0.05).Conclusions The ROS/SIRT1 axis is involved in hyperoxia-induced mitochondrial injury in BEAS-2B cells.
