Different concentrations of adapalene induce differentiation and apoptosis of SH-SY5Y cells
10.7499/j.issn.1008-8830.2310100
- VernacularTitle:不同浓度阿达帕林诱导SH-SY5Y细胞的分化及凋亡
- Author:
Na-Na LIU
1
;
Jun-Jiao ZHANG
;
Fan ZHANG
;
Cong-Ying WU
;
Yu-Wu JIANG
Author Information
1. 北京大学第一医院儿科,北京 100034;儿科遗传性疾病分子诊断与研究北京市重点实验室,北京 100009
- Keywords:
Adapalene;
Differentiation;
Apoptosis;
SH-SY5Y cell
- From:
Chinese Journal of Contemporary Pediatrics
2024;26(3):282-288
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different concentrations of adapalene on the morphology and functions of neuroblastoma cell line SH-SY5Y,as well as its role in inducing cell differentiation and apoptosis.Methods SH-SY5Y cells were divided into control group,low concentration(0.1 pM and 1 pM)adapalene groups,and high concentration(10 μM)adapalene group.Time-lapse microscopy was used to observe the morphological changes of SH-SY5Y cells.Immunofluorescence staining was performed to detect the expression of neuronal specific marker βⅢ-tubulin and mature neuronal marker neurofilament heavy polypeptide(NFH).Multi-electrode array was used to record the electrophysiological features of SH-SY5Y cells.Cell apoptosis was evaluated using a cell apoptosis detection kit.Results Low concentrations of adapalene promoted the formation of neurite outgrowth in SH-SY5Y cells,with the neurites interconnected to form a network.Spontaneous discharge activity was observed in SH-SY5Y cells treated with low concentrations of adapalene.Compared to the control group,the expression of βⅢ-tubulin and NFH increased in the 1 μM adapalene group,while the level of cell apoptosis increased in the high concentration adapalene group(P＜0.05).Conclusions Low concentrations of adapalene can induce differentiation of SH-SY5Y cells into mature functional neurons,while high concentrations of adapalene can induce apoptosis in SH-SY5Y cells.[Chinese Journal of Contemporary Pediatrics,2024,26(3):282-288]
