Core-shell Electrospining Injectable Drug Delivery System for Lung Cancer Treatment Study
10.11969/j.issn.1673-548X.2024.10.010
- VernacularTitle:芯壳静电纺丝可注射递药系统用于肺癌的治疗研究
- Author:
Cuiting GAO
1
;
Ze HE
;
Jiawei XING
Author Information
1. 610041 成都,四川大学华西口腔医院、口腔疾病防治全国重点实验室、国家口腔医学中心、国家口腔疾病临床医学研究中心
- Keywords:
Non-small cell lung cancer;
Sequential chemotherapy;
Electro spinning;
Short fiber;
Core-shell structure
- From:
Journal of Medical Research
2024;53(10):46-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a core-shell electrostatically spun short fiber capable of sequential drug release and its suspen-sion for local injection use,and to observe the effects of the short fiber on human non-small cell lung cancer cell line A549 and its trans-planted tumor in nude mice.Methods The structure and drug release characteristics of different(SF-HD:shell hydroxycamptothecin,core adriamycin;SF-DH:shell adriamycin,core hydroxycamptothecin)staple fibers were observed.A549 cells were treated with each group of staple fibers,and real-time cellular analysis and flow technology were used to observe growth and apoptosis.Nude mice were in-oculated with A549 cells above the right hind limb,and after successful modeling,they were randomly divided into blank,control,SF-HD,and SF-DH groups,and injected with 200ul of saline,blank short fiber suspension,SF-DH suspension,and SF-HD suspension above the center of the tumor,respectively.The day of injection was 0 day,and the volume of the tumors and the weight of the mice were measured every two days and plotted as a curve.After 5,10 and 21 days of injection,tumor tissues were taken and observed by HE stai-ning.Results Compared with SF-DH,SF-HD had more obvious effects in inhibiting the growth and promoting the apoptosis of A549 cells and their transplanted tumors.Conclusion The SF-HD group had a better inhibitory effect on A549 cells and their transplanted tumors.The slow-release system of electrostatically spun short fibers prepared in this study can achieve the effect of slow controlled re-lease over time and is a promising drug delivery system for research.
