Effect of lncRNA THOR Knockdown on Glucose Metabolic Reprogramming and Metastasis Ability of Colorectal Cancer Cells
10.11969/j.issn.1673-548X.2024.08.019
- VernacularTitle:敲低长链非编码RNA THOR对结直肠癌细胞糖代谢重编程和转移能力的影响
- Author:
Ziwei NIU
1
;
Yong ZHANG
;
Lu LI
Author Information
1. 450000 郑州大学;450007 郑州大学附属郑州中心医院消化内科
- Keywords:
LncRNA THOR;
Colorectal cancer cells;
Metastasis;
Glucose metabolism reprogramming;
ALDOC
- From:
Journal of Medical Research
2024;53(8):96-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of long non-coding RNA(lncRNA)THOR on glucose metabolism reprogram-ming,migration and invasion of colorectal cancer(CRC)cells,and its targeted regulatory relationship with fructose-1,6-bisphosphate aldolase C(ALDOC).Methods Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of THOR in CRC cell lines.THOR specific siRNA was transfected into HCT116 and SW480 cells to silence the expression of THOR,and the effects of THOR on the invasion and migration ability of CRC cells was detected by cell scratch assay and Transwell assay.The effect of THOR on glucose metabolic reprogramming in CRC cells was investigated by measuring the glucose intake and pyruvate pro-duction in CRC cells,and the expression levels of ALDOC mRNA and protein were detected by RT-qPCR and western blot.Results The results of RT-qPCR showed that the expression levels of THOR in HCT116 and SW480 cells were significantly higher than those in normal human intestinal epithelial cells NCM460.After the silencing of THOR,the cell scratch assay and Transwell assay showed that the migration and invasion ability of HCT116 and SW480 cells were inhibited,and the glucose uptake and pyruvate yield were decreased.The results of RT-qPCR and western blot showed that the expression levels of ALDOC mRNA and protein were significantly down-regulated,the difference was statistically significant(P＜0.05).Conclusion lncRNA THOR is highly expressed in CRC cells,which may affect the glucose metabolism reprogramming by targeting ALDOC to promote the migration and invasion of CRC cells.
