CeFe nanofibrous carbon nanozyme integrated with smartphone for the point-of-care testing of norfloxacin in water
10.1016/j.jpha.2024.101023
- Author:
Liu YUE
1
;
Cai TAIMEI
;
Chen SEN
;
Wen TAO
;
Peng HAILONG
Author Information
1. Key Laboratory of Natural Microbial Medicine Research of Jiangxi Province,School of Life Science,Jiangxi Science and Technology Normal University,Nanchang,330013,China;School of Chemistry and Chemical Engineering,Nanchang University,Nanchang,330031,China
- Keywords:
CeO2 nanoparticles;
Peroxidase-like activity;
Nanofibrous carbon microspheres;
Point-of-care testing;
Norfloxacin
- From:
Journal of Pharmaceutical Analysis
2024;14(10):1505-1513
- CountryChina
- Language:Chinese
-
Abstract:
The overuse of antibiotics has led to the severe contamination of water bodies,posing a considerable hazard to human health.Therefore,the development of an accurate and rapid point-of-care testing(POCT)platform for the quantitative detection of antibiotics is necessary.In this study,Cerium oxide(CeO2)and Ferrosoferric oxide(Fe3O4)nanoparticles were simultaneously encapsulated into N-doped nanofibrous carbon microspheres to form of a novel nanozyme(CeFe-NCMzyme)with a porous struc-ture,high surface area,and N-doped carbon material properties,leading to a considerable enhancement of the peroxidase(POD)-like activity compared with that of the CeO2 or Fe3O4 nanoparticles alone.The POD-like activity of CeFe-NCMzyme can be quenched using L-Cysteine(Cys)and subsequently restored by the addition of a quinolone antibiotic(norfloxacin,NOR).Therefore,CeFe-NCMzyme was used as a colorimetric sensor to detect NOR via an"On-Off"model of POD-like activity.The sensor possessed a wide linear range of 0.05-20.0 μM(R2=0.9910)with a detection limit of 35.70 nM.Furthermore,a smartphone-assisted POCT platform with CeFe-NCMzyme was fabricated for quantitative detection of NOR based on RGB analysis.With the use of the POCT platform,a linear range of 0.1-20.0 μM and a detection limit of 54.10 nM were obtained.The spiked recoveries in the water samples were ranged from 97.73％to 102.01％,and the sensor exhibited good accuracy and acceptable reliability.This study provides a portable POCT platform for the on-site and quantitative monitoring of quinolone antibiotics in real samples,particularly in resource-constrained settings.
