Effects of nano-polymer dendrimer-N-acetyl cysteine on retinal revascularization
10.13389/j.cnki.rao.2024.0101
- VernacularTitle:树枝状大分子N-乙酰半胱氨酸纳米聚合物对视网膜血管重建的作用
- Author:
Jing YANG
1
;
Yun QI
Author Information
1. 710061 陕西省西安市,西安交通大学第一附属医院健康医学科
- Keywords:
dendrimer;
N-acetyl cysteine;
oxygen-induced retinopathy;
revascularization;
neovascularization;
Delta-like ligand 4/Notch signaling pathway
- From:
Recent Advances in Ophthalmology
2024;44(7):526-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of the reactive oxygen species(ROS)inhibitor Dendrimer and N-acetyl cysteine(NAC)polymer(D-NAC)on retinal revascularization and its mechanism by inhibiting the expression of ROS in mice with oxygen-induced retinopathy(OIR).Methods The C57BL/6J mice were randomly divided into three groups:normal control group(NC group),OIR+Dendrimer group(OIR models+intravitreal injection of Dendrimer),and OIR+D-NAC group(OIR models+intravitreal injection of D-NAC).The mice were exposed to 75％oxygen from postnatal day 7(P7)to P1 2 and then to 21％oxygen from P1 2 to P1 7 to establish OIR models.The concentration of glutathione(GSH)was detected by GSH assay.The areas of retinal vessel occlusion(VO)and pathological neovascularization(NV)were quantitatively compared between the two OIR groups after fluorescence staining of the retina.The mRNA levels of retinal NV-related factors in mice between the two OIR groups were detected by quantitative reverse transcription polymerase chain reaction(qRT-PCR).The protein expression of retinal vascular endothelial growth factor(VEGF)in mice between the two OIR groups was detected by enzyme-linked immunosorbent assay(ELISA).The mRNA levels of Delta-like ligand 4(DLL4)/Notch pathway-associated factors in mice between the two OIR groups were detected by qRT-PCR.Results GSH assay results showed that there was no statistically significant difference in the retinal GSH level of mice between the NC group and the OIR+Dendrimer group on P12,P13,P14,P16,and P17(all P＞0.05).Compared with the OIR+Den-drimer group,the retinal GSH level of mice in the OIR+D-NAC group increased on P16 and P17(both P＜0.05).The reti-nal flat-mount staining results showed that compared with the OIR+Dendrimer group,the areas of VO and pathological NV in the OIR+D-NAC group decreased on P17(both P＜0.05).qRT-PCR results showed that on P16,compared with the OIR+Dendrimer group,the mRNA levels of fibroblast growth factor,angiogenin,VEGF,VEGF receptor 2 and erythropoie-tin decreased in the OIR+D-NAC group(all P＜0.05);ELISA results showed that compared with the OIR+Dendrimer group,the protein expression of VEGF decreased in the OIR+D-NAC group(P＜0.01).qRT-PCR results showed that compared with the OIR+Dendrimer group,the mRNA levels of DLL4/Notch pathway-associated factors such as Hey1,NRARP,DLL4,Notch1,Hes1 and Hey2 decreased on P16 in the OIR+D-NAC group(all P＜0.01).Conclusion ROS inhibitor D-NAC promotes physiological NV formation in OIR by inhibiting the DLL4/Notch signaling pathway,and restrains the pathological NV formation.
