Effect of asiaticoside on the repair of human corneal epithelial cell injury in-duced by high glucose
10.13389/j.cnki.rao.2024.0070
- VernacularTitle:积雪草苷在高糖诱导人角膜上皮细胞损伤修复中的作用及机制研究
- Author:
Xiaowen ZHI
1
;
Bing LI
Author Information
1. 121000 辽宁省锦州市,锦州医科大学
- Keywords:
diabetic keratopathy;
asiaticoside;
Wnt/β-catenin pathway;
damage repair
- From:
Recent Advances in Ophthalmology
2024;44(5):360-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and mechanism of asiaticoside(AS)on the repair of high glucose(HG)induced damage to human corneal epithelial cells(HCECs).Methods HCECs were cultured in vitro.The CCK-8 assay was used to detect the effects of different concentrations of glucose and AS on the proliferation activity of HCECs,and the optimal HG concentration(150 mmol·L-1)and AS concentration(20 μmol·L-1)were selected for subsequent experi-ments.HCECs were divided into the blank control group(NC group)(only added with culture medium DMEM),HG group(added with 150 mmol·L-1 glucose),AS group(added with 20 μmol·L-1 AS),and HG+AS group(added with 150 mmol·L-1 glucose and 20 μmol·L-1 AS).The proliferation activity of cells in each group at 24,48,and 72 hours was de-tected by the CCK-8 assay,respectively.The migration ability of cells in each group at 24 hours was detected by the cell scratch assay.TUNEL staining was used to detect the apoptosis rate of cells in each group;RT-PCR was used to detect the mRNA expression of Wnt1,β-catenin,and CyclinD1 in each group of cells;and Western blot was used to detect the protein expression of Wnt1,β-catenin,and CyclinD1 in each group of cells.Results Compared with the NC group,the prolifer-ation activity of cells in the HG group significantly decreased at 24,48,and 72 hours(all P＜0.001).Compared with the HG group,the proliferation activity of cells in the HG+AS group significantly increased at 24,48,and 72 hours(all P＜0.001).Compared with the NC group,the apoptosis rate of cells in the HG group increased markedly(P＜0.001).Com-pared with the HG group,the apoptosis rate of cells in the HG+AS group decreased markedly(P＜0.01).Compared with the NC group,the migration ability of cells in the HG group was significantly inhibited(P＜0.000 1).Compared with the HG group,the migration ability of cells in the HG+AS group was significantly enhanced(P＜0.000 1).Compared with the NC group,the mRNA expression levels of Wnt1,β-catenin,and CyclinD1 in the HG group were significantly reduced(all P＜0.05).Compared with the HG group,the mRNA expression levels of Wnt1,β-catenin,and CyclinD1 in the HG+AS group increased notably(all P＜0.001).Compared with the NC group,the protein expression levels of Wnt1,β-catenin,and CyclinD1 in the HG group decreased notably(all P＜0.01).Compared with the HG group,the protein expression lev-els of Wnt1,β-catenin,and CyclinD1 in the HG+AS group increased remarkably(all P＜0.01).Conclusion AS can alleviate HCEC damage caused by HG,promote cell proliferation and migration,and inhibit cell apoptosis.This effect may be achieved by activating the Wnt/β-catenin signaling pathway.
