The Effect and Mechanism of MVP on the Function of Human Renal Cancer Cells
10.3870/j.issn.1672-0741.24.01.002
- VernacularTitle:MVP对人肾癌细胞功能的影响及机制研究
- Author:
Hainan ZHAO
1
;
Lina ZHOU
Author Information
1. 锦州医科大学附属第一医院肾内科,锦州 121000
- Keywords:
major vault protein;
renal cell carcinoma;
proliferation;
migration;
invasion;
PI3K/Akt/mTOR sig-naling pathway
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2024;53(5):629-634,680
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of knocking down the expression of major vault protein(MVP)on the prolifer-ation,migration,and invasion of renal cancer cells and its possible mechanisms of action.Methods The basic expression levels of the MVP gene in human renal tubular epithelial cells(HK2)and human renal cancer cell lines(786-O,769-P,and ACHN)were detected by qRT-PCR and Western blotting,and the cell lines with significantly high MVP expression were screened as experi-mental cell lines.The effects of MVP knockdown on the proliferation,migration and invasion of human renal cancer cells were observed via cell colony formation,EdU incorporation,cell cycle,cell scratch and Transwell assays.The expression of PI3K/Akt/mTOR pathway proteins was detected via Western blotting.The mechanism by which the MVP gene regulates the biologi-cal behavior of renal cancer cells was determined by a pathway activator.Results The results of qRT-PCR and Western blotting showed that,compared with those in HK2 cells,the MVP mRNA and protein expression levels in 786-O,769-P and ACHN cells were significantly increased(all P＜0.05),especially in 769-P cells.After MVP was knocked down in 769-P cells,their ability to form colonies was significantly weakened(P＜0.01).The number of EdU-positive cells decreased significantly(P＜0.01).The proportion of cells in the S phase decreased(P＜0.01),whereas the proportion of cells in the G2 phase increased(P＜0.01).The migration and invasion abilities of the cells were significantly inhibited(all P＜0.01).The protein expression of p-PI3K,p-Akt and p-mTOR decreased(all P＜0.01).The protein expression of p-PI3K,p-Akt and p-mTOR increased when MVP was knocked down and when the PI3K activator 740Y-P was given at the same time(all P＜0.01).The inhibition of cell prolifera-tion,migration and invasion caused by MVP knockdown was also reversed by 740Y-P(all P＜0.01).Conclusion Knocking down the MVP gene can inhibit the proliferation,migration and invasion of human renal cancer cells,which may be achieved by blocking the activation of the PI3K/Akt/mTOR signaling pathway.
