Effect of transient receptor potential channel 5 on myocardial pyroptosis in intermittent hypoxia
10.3969/j.issn.1006-5725.2024.12.005
- VernacularTitle:瞬时受体电位通道5对间歇性低氧致心肌焦亡的影响
- Author:
Xuan QIU
1
;
Yishajiang SHAREZATI
;
Yulan CHEN
;
Mengmeng WANG
;
Yu LI
;
Tulahong GULINAZI
;
Abuhan ZUBAIDAN
;
Abulizi ALIYA
;
Xingchen WANG
Author Information
1. 新疆医科大学第一附属医院 心血管病中心高血压科(乌鲁木齐 830000)
- Keywords:
transient receptor potential channel 5;
inflammatory factor;
pyroptosis;
intermittent hypoxia;
OSAHS
- From:
The Journal of Practical Medicine
2024;40(12):1637-1642
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of TRPC5 on intermittent hypoxia-induced myocardial pyroptosis.Methods TRPC5 knock out(TRPC5-/-)and SD rats were each randomly divided into chronic intermit-tent hypoxia group(CIH group)and normoxia group(Control group)(n=6 for each group).The myocardial fibrosis in rats was visualized by Masson staining,the levels of serum inflammatory factors were measured by ELISA and the relative expression levels of TRPC 5 and pyroptosis-related proteins were determined by Western blotting.Results Masson staining showed that the collagen volume fraction in the TRPC5-/--CIH group was higher than that in the TRPC5-/--Control group and lower than that in the WT-CIH group.The ELISA results showed that the serum levels of IL-1,IL-6,and TGF-β in the TRPC5-/--CIH group were higher than those in the TRPC5-/--Control group and lower in the WT-CIH group.Western blotting results showed that the relative expression levels of pyroptosis related proteins caspase-1,NLRP3,GSDMD,and GSDMD-N in the TRPC5-/--CIH group were higher than those in the TRPC5-/--Control group and lower than those in the WT-CIH group.Conclusion TRPC5 deficiency alleviates myocardial pyroptosis and myocardial fibrosis induced by hypoxia.
