Dexmedetomidine alleviates propofol induced learning and memory impairment in rats at developmental stage by regulating AKAP150
10.3969/j.issn.1006-5725.2024.12.002
- VernacularTitle:右美托咪定通过调控AKAP150减轻异丙酚诱导的发育期大鼠学习记忆障碍
- Author:
Di WANG
1
;
Jian YANG
;
Xiang HE
Author Information
1. 贵州医科大学麻醉学院(贵阳 550004)
- Keywords:
dexmedetomidine;
propofol;
A kinase anchor protein 150;
learning and memory;
pyroptosis
- From:
The Journal of Practical Medicine
2024;40(12):1619-1624
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of A kinase anchor-protein 150(AKAP150)in alleviating learning and memory impairment induced by propofol in rats at developmental stage.Methods 80 SD rats aged 7 days were randomly divided into a control group(Con group),propofol group(Pro group),dexmedetomidine pre-administration group(DP group),and AKAP150 adenovirus plus DP group(ADP group)(n=20).The Con group was injected with equal volume normal saline.The Pro group was injected with propofol of 50 mg/kg twice,and the DP group was injected with dexmedetomidine of 25 μg/kg plus propofol of 50 mg/kg.The ADP group was treated with adenovirus to construct an AKAP150 knockout model.Expression levels of AKAP150,PKA,NLRP3,GSDMD,IL-1β and IL-18 in hippocampus were detected by Western blot.The ultrastructure of hippocampus was observed by transmission electron microscopy.Results After propofol treatment,hippocampal cell membrane split and pores formed,expressions of AKAP150 and PKA were down-regulated(P<0.05),while expressions of NLRP3,GSDMD,IL-1β and IL-18 were up-regulated(P<0.05),and the frequency of crossing the platform was decreased(P<0.05).After pre-administration of dexmedetomidine,the cell membrane structure of hippocampus was basically normal,expressions of AKAP150 and PKA were up-regulated(P<0.05),expressions of NLRP3,GSDMD,IL-1β and IL-18 were down-regulated(P<0.05),and the frequency of crossing the platform was increased(P<0.05).Conclusions Dexmedetomidine may enhance the activity of PKA and inhibit expressions of NLRP3,GSDMD,IL-1β and IL-18 by activating expression of AKAP150,thereby alleviating propofol-induced pyroptosis in hippocampal tissues and improving learning and memory impairment in rats at developmental stage.
