Potential application value of FilmArray ? meningitis/encephalitis panel in children with suspected central nervous system infections
10.3760/cma.j.cn101070-20230922-00220
- VernacularTitle:脑炎/脑膜炎多重病原体核酸联合检测试剂盒在儿童疑诊中枢神经系统感染中的潜在应用价值
- Author:
Xue NING
1
;
Muhan LI
;
Xin GUO
;
Huili HU
;
Lingyun GUO
;
Bing HU
;
Tianming CHEN
;
Gang LIU
Author Information
1. 国家儿童医学中心，首都医科大学附属北京儿童医院感染科，儿科重大疾病研究教育部重点实验室，北京 100045
- Keywords:
Child;
Central nervous system infection;
Pathogen;
Rapid diagnosis
- From:
Chinese Journal of Applied Clinical Pediatrics
2024;39(7):519-522
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the clinical application and effect of FilmArray ? meningitis/encephalitis (ME) panel in identifying pathogens of central nervous system (CNS) infections in children. Methods:Molecular biology study.Cerebrospinal fluid (CSF) samples were prospectively obtained through lumbar puncture from children with suspected CNS infections admitted to the Department of Infectious Diseases at Beijing Children′s Hospital, Capital Medical University from May to November 2019.These samples were subjected to both routine clinical pathogen testing and FilmArray ME panel testing.Polymerase chain reaction was used to validate all samples.Independent sample t-test or Mann-Whitney U-test was used for comparative analysis of the results and influence factors obtained by the two detection methods. Results:A total of 113 cases of suspected CNS infections were enrolled.Routine clinical testing yielded 17 cases, with a positive rate of 15.0%, including 4 positive CSF cultures, with a positivity rate of 3.5%.FilmArray ME panel detected 23 positive cases, with a positive rate of 20.4%.FilmArray ME panel detected bacteria in 7 cases, viruses in 13 cases, fungi in 1 case, and both viruses and bacteria in 2 cases.Among the common pathogens detected, FilmArray ME panel obtained the results on average 2.7 days in advance.Conclusions:Compared with CSF culture, FilmArray ME panel has the advantages of shorter detection period, higher positive detection rate, and higher virus detection rate.
