Correlation between the Expression of LNMAS mRNA and CLDN4 mRNA Levels in Cervical Cancer Tissue and Epithelial-Mesenchymal Transition and Clinical Significance
10.3969/j.issn.1671-7414.2024.06.015
- VernacularTitle:宫颈癌组织中LNMAS mRNA,CLDN4 mRNA水平表达与上皮间质转化的相关性及临床意义研究
- Author:
Chunwen LIU
1
;
Yue WANG
;
Xinyue LIU
;
Wenbo XU
;
Zhimin SUN
Author Information
1. 沧州市妇幼保健院妇女体检与健康管理科,河北 沧州 061000
- Keywords:
cervical cancer;
long non-coding RNA lymph node metastasis-associated suppressor;
tight junction protein claudin 4;
epithelial-mesenchymal transition
- From:
Journal of Modern Laboratory Medicine
2024;39(6):90-95
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between the expression of long non-coding RNA lymph node metastasis-associated suppressor(LNMAS)mRNA and tight junction protein claudin 4(CLDN4)mRNA and epithelial-mesenchymal transition(EMT)in cervical cancer and the clinical significance.Methods A total of 96 patients with cervical cancer who were first diagnosed and treated at Cangzhou Maternal and Child Health Hospital from June 2018 to June 2020 were selected.Real-time fluorescent quantitative PCR was used to detect the expressions of LNMAS mRNA,CLDN4 mRNA and EMT-related genes.Pearson correlation analysis was used to analyze the correlation between LNMAS mRNA,CLDN4 mRNA and VIM mRNA,E-cad mRNA,N-cad mRNA.The impact of LNMAS mRNA and CLDN4 mRNA expression on the 3-year survival rate of cervical cancer patients was analyzed using the K-M curve.Cox regression was used to analyze prognostic factors for cervical cancer.Results Compared to adjacent tissues,the expressions of LNMAS mRNA(1.13±0.28 vs.1.97±0.37)and E-cadherin(E-cad)mRNA(1.02±0.33 vs 2.29±0.56)in cervical cancer tissues were lower(t=17.738,19.144),while CLDN4 mRNA(3.14±0.52 vs 1.19±0.28),N-cadherin(N-cad)mRNA(2.60±0.36 vs 1.02±0.33)and Vimentin(VIM)mRNA(2.84±0.46 vs 1.25±0.33)were higher(t=32.351,26.951,27.518),with significant differences(all P<0.05).LNMAS mRNA showed a positive correlation with E-cad mRNA(r=0.712,P<0.05),but a negative correlation with VIM mRNA and N-cad mRNA in cervical cancer tissues(r=-0.654,-0.589,all P<0.05).Additionally,the expression of CLDN4 mRNA was negatively correlated with E-cad mRNA(r=-0.668,P<0.05),but positively correlated with VIM mRNA and N-cad mRNA(r=0.714,0.749,all P<0.05).Compared with patients with FIGO stage ⅠA～ⅠB1 and no lymph node metastasis,patients with FIGO stage ⅠB2-ⅡA and lymph node metastasis exhibited a lower LNMAS mRNA expression and a higher CLDN4 mRNA expression with significant differences(t=12.288,10.228,13.636,11.771).Comparison Log-Rank x2 square test,the 3-year overall survival rates for high and low expression groups of LNMAS mRNA were 92.00％(46/50)and 60.87％(28/46),respectively;the 3-year overall survival rates for high and low expression groups of CLDN4 mRNA were 59.18％(29/49)and 95.74％(45/47),respectively,and the difference between the two groups were significant(log-Rank x2=11.030,15.600,all P<0.001).FIGO stage ⅠB2～ⅡA(HR=1.119,95％CI:1.148～1.830),lymph node metastasis(HR=1.442,95％CI:1.124～1.850)and CLDN4 mRNA(HR=1.637,95％CI:1.124～1.850)were risk factors affecting the prognosis of cervical cancer patients,while LNMAS mRNA(HR=0.637,95％CI:0.465～0.873)was a protective factor(all P<0.05).Conclusion The expression of LNMAS mRNA is decreased,while the expression of CLDN4 mRNA is increased in cervical cancer.The expressions of LNMAS mRNA and CLDN4 mRNA were associated with the process of EMT,which may be markers for evaluating the prognosis of patients with cervical cancer.
