Plasma ctDNA SCNM1 Methylation Level and Its Clinical Prognostic Value in Hepatocellular Carcinoma Patients
10.3969/j.issn.1671-7414.2024.06.003
- VernacularTitle:肝癌患者血浆ctDNA SCNM1甲基化水平检测及与临床预后评估价值研究
- Author:
Juan WANG
1
;
Liu YANG
;
Jiayun LIU
Author Information
1. 空军军医大学第一附属医院检验科,西安 710032
- Keywords:
circulating tumour DNA;
sodium channel modifier 1;
hepatocellular carcinoma;
methylation
- From:
Journal of Modern Laboratory Medicine
2024;39(6):18-22,36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between prognostic value and methylated expression level of circulating tumour DNA(ctDNA)sodium channel modifier 1(SCNM1)in plasma of patients with hepatocellular carcinoma(HCC).Methods A total of 67 patients with confirmed HCC admitted to the First Affiliated Hospital of AFMU from June 2018 to December 2020 were selected as the research objects.At the same time,50 healthy volunteers were selected as the control group.Their clinical and pathological data were collected and compared.The methylation level of SCNM1 was detected by droplet digital PCR(ddPCR).The differences of alpha fetoprotein(AFP),cereal third transaminase(ALT),aspartate transaminase(AST),ctDNA,SCNM1 between HCC group and control group were analyzed.Multivariate COX regression risk model was performed to analyze the risk factors of HCC patients.Receiver operating characteristic(ROC)curve was used to analyze the sensitivity and specificity of SCNM1 methylation levels methylation levels.Results AFP[351.00(14.90,1 210.00)ng/ml],ctDNA[57.65(15.79,171.90)ng/ml]and the methylated SCNM1 levels[13.23%(4.36%,26.61%)]in plasma in HCC group were higher than those in control group[2.90(2.20,5.10)ng/ml,15.75(12.85,21.07)ng/ml,4.69%(3.30%,6.68%)],and the differences were statistically significant(Z=-7.18,-5.00,-4.77,all P<0.05).High level of SCNM1 methylation was found in HCC patients with tumor lesion diameter>2cm and distant metastasis(x2=5.01,38.85,all P<0.05).The median survival was shorter in the high-methlated SCNM1 group than that in the low-methylated SCNM1 group(13.31 month vs 24.44 month)with significant difference(Log rankx2=4.141,P=0.04).High SCNM1 methylation level was independent risk factor for HCC patients survival(95%CI:2.449～62.716,P=0.002).The ROC curve showed prognostic value of increased methylated SCNM1 was 0.716(95%CI:0.583～0.848),and the sensitivity and specificity were 63.0%and 81.1%,respectively.When combined with plasma SCNM1 methylation level,ctDNA and AFP,the AUC(95%CI)of HCC progression was 0.747(0.618～0.875),and the sensitivity and specificity were 55.6%and 91.9%,respectively,which has potential application value for dynamic monitoring of liver cancer patients.Conclusion The high level of methylated SCNM1 in plasma indicates the HCC poor prognosis,and serves as an independent determinant of patient survival.Methylated SCNM1,ctDNA combined with AFP detection can serve as prognostic markers.
