Performance Evaluation of Automatic Magnetic Bead Extraction of Serum Testosterone by Liquid Chromatography-tandem Mass Spectrometry
10.3969/j.issn.1671-7414.2024.04.033
- VernacularTitle:自动化磁珠法提取血清睾酮应用液相色谱串联质谱法检测的性能评价
- Author:
Li LI
1
;
Yaling HUANG
Author Information
1. 重庆市开州区人民医院检验科,重庆 405400
- Keywords:
automated magnetic specimen preparation;
liquid chromatography-tandem mass spectrometry;
serum testosterone
- From:
Journal of Modern Laboratory Medicine
2024;39(4):180-185
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an automated magnetic specimen preparation(MSPE)extraction combined with liquid chromatography tandem mass spectrometry(LC-MS/MS)in the detection of testosterone(T)in human serum,and evaluate its performance.Methods A total of 150 patients treated in Kaizhou District People's Hospital of Chongqing from April to June 2023 were selected as the study objects.MSPE combined with LC-MS/MS method was used to detect the serum testosterone of the patients.Excluding the abnormalities of serum hemolysis,chylus and jaundice,the specificity,linearity,limit of quantification,recovery rate,precision and matrix effect of the method were evaluated.The results of chemiluminescence immunoassay(CLIA)and MSPE combined with LC-MS/MS were compared.Results The detection limit and quantitation limit of MSPE combined with LC-MS/MS method for the detection of testosterone were 0.005 ng/ml and 0.05 ng/ml,respectively,and the correlation was good in the range of 0.05～15 ng/ml with r＞0.99.Intra-lot,inter-lot and total precision were less than 15％.The recovery rate was 105.5％～106.2％.The matrix effect of serum internal standard was close to that of analyte.Compared with CLIA method,the correlation between the two methods was better(r=0.997 1),and the relative percentage deviation was less than 15％.Conclusion The combination of MSPE extraction and LC-MS/MS detection of testosterone in human serum has good performance,which can meet the quantitative analysis requirements of testosterone in human serum samples.
