Study on the Correlation between Sputum Metagenomics and Microbiota in Elderly Patients with Severe Pneumonia at Different Blood Glucose Levels
10.3969/j.issn.1671-7414.2024.04.021
- VernacularTitle:老年重症肺炎不同血糖水平患者痰液宏基因组学与菌群相关性研究
- Author:
Chang ZHAO
1
;
Fang XIE
;
Ying XIANG
Author Information
1. 西部战区总医院干部病房三科二病区,成都 610083
- Keywords:
blood glucose;
severe pneumonia in the elderly;
sputum microbiota;
metagenomics
- From:
Journal of Modern Laboratory Medicine
2024;39(4):116-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the correlation between sputum metagenomics and microbiota in elderly patients with severe pneumonia at different blood glucose levels.Methods A total of 158 elderly patients with severe pneumonia who visited Western War Zone General Hospital from May 2020 to May 2023 were selected as the study subjects.All patients were divided into a hyperglycemic group(blood glucose＞7.8 mmol/L,n=68)and a normal group(blood glucose was 3.9～7.8 mmol/L,n=90)based on their blood glucose levels at admission.Metagenomic sequencing on the sputum microbiota of all patients was performed,and the species composition of the patient's sputum microbiota was analyzed.The differences in alpha diversity,beta diversity,flora abundance,microbial network,gene function and other aspects of sputum flora between the hyperglycemic group and the normal group were compared.Results The Shannon index,Simpson index,and Bray-Curtis distance of the hyperglycemic group were 2.01±0.63,0.60±0.03 and 0.52±0.09,respectively,which were lower than the 2.67±0.45,0.64±0.05 and 0.61±0.07 of the normal blood glucose group,with significant differences(t=7.681,5.847,7.071,all P＜0.05).At the phylum level,the relative abundances of Firmicutes and Actinobacteria in the hyperglycemic group were 32.54％±16.91％and 7.58％±4.36％,respectively,and they were lower than 58.22％±7.33％and 9.80％±3.94％in the normal blood glucose group,with significant differences(t=12.901,3.349,all P＜0.05).At the genus level,the relative abundances of Fecal Bacillus,Microbacterium and Gastroma genera in the hyperglycemic group were 1.67％±0.72％,0.24％±0.09％and 0.29％±0.13％,respectively,which were lower than 28.43％±5.89％,1.02％±0.45％and 1.85％±1.34％in the normal blood glucose group,the relative abundance of Enterococcus species was 32.77％±15.80％,which was higher than the 21.46％±6.21％in the normal blood glucose group,and the differences were statistically significant(t=37.227,14.072,9.559,6.192,all P＜0.05).The microbial network of sputum microbiota in patients with hyperglycemia was more fragile.The functional genes with higher relative abundance in the hyperglycemic group were the structural components of ribosomes and cell membranes.The relatively abundant signaling pathways were glycolysis Ⅰ pathway(glucose metabolism pathway),glycolysis Ⅱ pathway(fructose metabolism pathway),and methylerythritol phosphate Ⅱ pathway.Conclusion Blood glucose was an important factor affecting the diversity of sputum microbiota in elderly patients with severe pneumonia,and there were significant differences in the structure of sputum microbiota among elderly patients with severe pneumonia with different blood glucose levels.
