Inhibition of zona incerta GABAergic neurons regulates lipopolysaccharide induced motor dysfunction in mice
10.16557/j.cnki.1000-7547.2024.03.010
- VernacularTitle:未定带GABA能神经元活性降低介导脂多糖诱导的小鼠运动能力受损
- Author:
Hongquan ZHANG
1
;
Xinyi WANG
;
Kun TONG
;
Jie HAN
;
Can GAO
Author Information
1. 徐州医科大学江苏省麻醉学重点实验室,徐州 221004
- Keywords:
lipopolysaccharide(LPS);
zona incerta(ZI);
GABAergic neuron;
neuroinflammation;
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(3):347-352
- CountryChina
- Language:Chinese
-
Abstract:
Objective:By activating gamma-aminobutyric acid(GABA)neurons in zona incerta(ZI),we observed the alleviation of motor ability impairment in mice treated with lipopolysaccharide(LPS),and investigate the function of GABA neurons in the process of motor function impairment in mice with neuroinflammation.Methods:The central nervous system infection model of C57BU6 mice was eatablished by intraperitoneal injection of LPS.The motor function were detected by open field test and running wheel test in mice.The expression of c-Fos in ZI neurons and ionized calci-um binding adaptor molecule 1(Iba-1)in glial cells were detected by immunofluorescence staining.rAAV-DIO-hM3Dq-mCherry was bilaterally injected into ZI of adult male VGAT-Cre mice through the stereotaxic technique.After three weeks with the virus sufficiently expressing in the neurons,the neuroinflammation model was made by intrabitoneal injection of LPS,and GABAergic neurons were activated by intrabitoneal injection of N-oxyclozapine(CNO)30 min before behavioral tests.The motor ability in mice were detected by open field test and running wheel test.Results:The results of open field experiment and wheel experiment showed that the activity of mice in LPS group was significantly decreased(P＜0.05)compared with the control group.The expression of ZI c-Fos positive protein in the brain of mice was significantly down-regulated with Iba-1 positive protein up-regulating in the ZI.The chemogenetic results showed that the activity of LPS mice was relieved with ZI GABAergic neurons being activated(P＜0.05).Conclusion:The activity of ZI GABAergic neurons was significantly decreased and the motor ability was impaired in mice with neuroin-flammation in the brain.Chemogenetic activation of ZI GABAergic neurons significantly improved the motor ability of inflammatory mice.
