Synthesis of polyacrylic acid-citrate-amorphous calcium phosphate and its effect on dentin remineralization
10.13591/j.cnki.kqyx.2024.11.001
- VernacularTitle:聚丙烯酸-柠檬酸盐-无定形磷酸钙的合成及其对牙本质再矿化的影响
- Author:
Yinying CHEN
1
;
Chen CHEN
Author Information
1. 南京医科大学附属口腔医院牙体牙髓科,口腔疾病研究与防治国家级重点实验室培育建设点,江苏省口腔转化医学工程研究中心,江苏 南京(210029)
- Keywords:
citrate;
amorphous calcium phosphate;
dentin remineralization
- From:
STOMATOLOGY
2024;44(11):801-805
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare and identify the polyacrylic acid-citrate-amorphous calcium phosphate composite(PAA-Cit-ACP),and analyze its effect on dentin remineralization.Methods PAA-Cit-ACP was prepared by co-precipitation and freeze-drying methods.The composite was characterized by FTIR,XRD,Zeta potential,SEM,TEM and SAED.SEM was used to analyze the effect of PAA-Cit-ACP on dentin remineralization.Cell cytotoxicity of PAA-Cit-ACP was evaluated by CCK-8 method.Results The charac-teristic peaks of carboxyl(1 567 cm-1 and 1 416 cm-1)and ACP(1 064 cm-1 and 563 cm-1)were simultaneously observed in the FT-IR spectrum,and the XRD and SAED results confirmed its amorphous state.The Zeta potential of PAA-Cit-ACP was-18.47 mV.PAA-Cit-ACP appeared as spherical nanoparticles according to SEM and TEM.In SEM images,a large amount of new minerals formed on the surface of demineralized dentin after PAA-Cit-ACP treatment.The CCK-8 result showed that PAA-Cit-ACP exhibited good bio-compatibility at a concentration of 250,500,and 1 000 μg/mL.Conclusion Citrate can co-stabilize supersaturated calcium phosphate solutions with polyacrylic acid,forming polyacrylic acid citrate amorphous calcium phosphate which can promote dentin remi-neralization.
