Effects of conditional knockout ceruloplasmin in astrocytes on brain iron metabolism of mice
10.16098/j.issn.0529-1356.2024.04.014
- VernacularTitle:星形胶质细胞敲除铜蓝蛋白对小鼠脑铁代谢的影响
- Author:
Hai-Yan LI
1
;
Zhong-Da LI
;
Li-Juan HE
Author Information
1. 承德医学院人体解剖学教研室,河北承德 067000;河北师范大学生命科学学院河北省动物生理生化与分子生物学重点实验室,石家庄 050024
- Keywords:
Ceruloplasmin;
Astrocyte;
Iron;
Cognition;
Western blotting;
Mouse
- From:
Acta Anatomica Sinica
2024;55(4):475-481
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of ceruloplasmin(CP)in brain iron metabolism.Methods We obtained glial fibrillary acidic protein(GFAP)-cre-mediated ceruloplasmin knockout mice(CPGFAP cKO mice)by crossing CPflox/flox micewithtransgenic GFAP-cre mice.Eighty-eight male mice aged 6 months were divided into four groups,WT,GFAP-cre,CPflox/flox and CPGFAP cKO.To observe whether astrocytic CP gene knockout specifically through genotype identification and immunofluorescent staining,and Western blotting method.The learning and memory function was detected by Morris water maze,brain iron levels in the cortex and hippocampus of mice were measured by inductively coupled plas mamass spectrometry(ICP-MS)and μ-probe X-ray fluorescence(μ-XRF),and iron contents in serum and liver were detected by iron kit.Results The mice of conditional knockout ceruloplasmin in astrocytes were obtained successfully by genotype identification,immunofluorescent staining and Western blotting method.The learning and memory ability was reduced in the water maze experiment,and iron contents decreased in the cortex and hippocampal regions of 6-month-old CPGFAP cKO group.While the level of serum iron and liver iron in the four groups did not change significantly.Conclusion The decline of learning and memory ability of mice with astrocytic CP gene knockout specifically is closely related to the decrease in brain iron.The main function of CP may be to help brain cells to absorb iron.
