Exploring the Mechanism of Bushen Yixin Tablets in the Treatment of Diabetic Nephropathy Based on Network Pharmacology and Cellular Experimental Validation
10.13359/j.cnki.gzxbtcm.2024.11.029
- VernacularTitle:基于网络药理学及细胞实验验证探讨补肾益心片治疗糖尿病肾病的作用机制
- Author:
Yan-Na YU
1
;
Liang-Liang WANG
;
Xin DONG
;
Shui-Fu TANG
Author Information
1. 广州中医药大学第一附属医院,广东 广州 510405;广东省中医临床研究院,广东 广州 510405
- Keywords:
Bushen Yixin Tablets;
diabetic nephropathy;
network pharmacology;
PI3K/AKT/BCL-2 pathway;
experimental validation
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(11):3016-3024
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of Bushen Yixin Tablets in the treatment of diabetic nephropathy(DN).Methods The key targets of Bushen Yixin Tablets for the treatment of DN were predicted by network pharmacology.A high-glucose-processed MPC-5 cell model was constructed,enzyme-linked immunosorbent assay(ELISA),real-time quantitative polymerase chain reaction(RT-qPCR)and Western Blot were applied to verify the potential action targets and pathways of Bushen Yixin Tablets for the treatment of DN.Results Twenty-four active ingredients were obtained from Bushen Yixin Tablets.There were 131 targets involved in the treatment of DN by Bushen Yixin Tablets,and the results of pathway enrichment analysis showed that the key targets might mainly focus on inflammatory pathways,lipids and atherosclerosis.The further experimental validation showed that,compared with the high glucose group,the secretion level of matrix metalloproteinase 1(MMP-1)in the supernatant of MPC-5 cells in the medium-and high-dose groups of Bushen Yixin Tablets medicated serum were increased(P＜0.001),and the secretion levels of tissue inhibitor of metalloproteinase 1(TIMP-1)and transforming growth factor β1(TGF-β1)were decreased(P＜0.001).Compared with the high glucose group,the protein and mRNA expression levels of MMP1 and BCL-2 in the high-dose group of Bushen Yixin Tablets medicated serum were increased(P＜0.001),and the protein and mRNA expression levels of phosphatidylinositol 3-kinase(PI3K)and protein kinase B(AKT)were decreased(P＜0.001).Conclusion Bushen Yixin Tablets may play a protective role against DN by inhibiting the proliferation of glomerular mesangial cells and promoting the degradation of extracellular matrix through PI3K/AKT/BCL-2 pathway.
