Bushen Jianpi Chinese Herbal Compound Activates AMPK Signaling Pathway to Inhibit Overweight in Estrogen-Deficient Mice
10.13359/j.cnki.gzxbtcm.2024.10.036
- VernacularTitle:补肾健脾中药复方激活AMPK信号通路抑制雌激素缺乏小鼠超重
- Author:
Shu-Jia XIE
1
;
Tao-Rui WU
;
Guo-Yi LI
;
Yuan-Hao HU
;
Xiao-Ling SHEN
;
Ying-Jie HU
Author Information
1. 广州中医药大学科技创新中心,广东广州 510405
- Keywords:
Zhenyuan Granules;
replenishing kidney and invigorating spleen;
overweight;
ovariectomy;
adipose differentiation;
adenylate-activated protein kinase(AMPK);
3T3-L1 cells;
mice
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(10):2769-2777
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the intervention effect and mechanism of Chinese herbal compound(Zhenyuan Granules)on overweight in mice caused by estrogen deficiency.Methods An ovariectomized female mouse model was established to observe the effects of intragastric 14 week administration of Zhenyuan Granules on body mass and fat accumulation in mice.The 3T3-L1 mouse preadipocyte model was constructed to observe the effect of Zhenyuan Granules intervention on adipogenic differentiation.The expressions of key genes/proteins regulating adipocyte formation and fat synthesis in mouse adipose tissue and 3T3-L1 cells was detected to explore the mechanism of lipid-lowering effect of Zhenyuan Granules in vivo and in vitro.Results Zhenyuan Granules significantly decreased the body mass gain(P＜0.01),perigonadal and inguinal fat indexes(P＜0.05),significantly inhibited the differentiation of 3T3-L1 preadipocytes into mature adipocytes(P＜0.01),significantly downregulated the expression levels of transcription factors peroxisome proliferators activated receptor γ(PPARγ)and sterol-regulatory element binding protein 1c(SREBP-1c)and enzymes acetyl-CoA carboxylase 1(ACC1)and fatty acid synthase(FAS)in adipose tissue and 3T3-L1 preadipocytes(P＜0.05),significantly enhanced the phosphorylation of AMP-activated protein kinase(AMPK)and ACC1(P＜0.05).Conclusion Zhenyuan Granules can inhibit the formation of adipocytes and improve the overweight of female mice caused by estrogen deficiency.The mechanism is related to the activation of AMPK signaling pathway.It is suggested that Bushen Jianpi Chinese herbal compound Zhenyuan Granules is helpful for body mass control in postmenopausal women.
