Anti-Tumor Effect and Mechanism of Yiqi Tongqiao Prescription on Nasopharyngeal Carcinoma in Nude Mice
10.13359/j.cnki.gzxbtcm.2024.09.029
- VernacularTitle:益气通窍方对鼻咽癌裸鼠的抑瘤作用及机制
- Author:
Xue-Yan CHANG
1
;
Ling ZHOU
;
Jia-Peng YANG
;
Jing-Dan LIU
Author Information
1. 黑龙江中医药大学,黑龙江哈尔滨 150040
- Keywords:
Yiqi Tongqiao Prescription;
nasopharyngeal carcinoma;
tissue inhibitor of metalloproteinase 1(TIMP-1);
matrix metalloproteinase 9(MMP-9);
transforming growth factor β1(TGF-β1);
vascular endothelial growth factor(VEGF);
Wnt-1/β-catenin pathway;
nude mice
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(9):2426-2432
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect and mechanism of Yiqi Tongqiao Prescription on nasopharyngeal carcinoma in nude mice.Methods Thirty-two nude mice were randomly divided into normal group,model group,Yiqi Tongqiao Prescription low-and high-dose groups,with eight mice in each group.Except for the normal group,the other groups of nude mice were used to construct a nasopharyngeal carcinoma model.After treatment,the tumor volume of nude mice was measured,the levels of serum transforming growth factor β1(TGF-β1)and vascular endothelial growth factor(VEGF)were detected by enzyme-linked immunosorbent assay(ELISA),the pathological morphology of nasal mucosa was observed by hematoxylin-eosin(HE)staining,the mRNA expressions of tissue inhibitor of metalloproteinase 1(TIMP-1)and matrix metalloproteinase 9(MMP-9)in nasal mucosa were detected by quantitative polymerase chain reaction(PCR),the protein expressions of Wnt-1 andβ-catenin in nasal mucosa were detected by Western Blot.Results Compared with the normal group,the tumor volume of nude mice in the model group was increased,the levels of serum TGF-β1 and VEGF were increased,the mRNA expression level of TIMP-1 in nasal mucosa was decreased,the mRNA expression level of MMP-9 was increased,and the protein expression levels of Wnt-1 and β-catenin in nasal mucosa were increased(all P＜0.05).HE staining results showed structural defects of nasal mucosa,and a large number of eosinophils and other inflammatory cell infiltration.Compared with the model group,the tumor volume of nude mice in the low-and high-dose groups of Yiqi Tongqiao Prescription was reduced,the levels of serum TGF-β1 and VEGF were decreased,the mRNA expression level of TIMP-1 in nasal mucosa was increased,the mRNA expression level of MMP-9 was decreased,and the protein expression levels of Wnt-1 and β-catenin in nasal mucosa were decreased(all P＜0.05)in a dose-dependent manner.HE staining results showed that the morphology of nasal mucosa cells was significantly improved.Conclusion Yiqi Tongqiao Prescription inhibits the activity of MMP-9 through the endogenous regulation of TIMP-1 to maintain the homeostasis of extracellular matrix in nasopharyngeal carcinoma,and inhibits the abnormal activation of Wnt/β-catenin signaling pathway and reduces the release of TGF-β1 and VEGF to reduce tumor angiogenesis,thereby playing a role in tumor inhibition for nude mice with nasopharyngeal carcinoma.
