Study on HPLC Aging Characteristic Chromatogram of Guangdong Citri Reticulatae Pericarpium
10.13359/j.cnki.gzxbtcm.2024.08.032
- VernacularTitle:广陈皮高效液相色谱陈化特征图谱研究
- Author:
Ming-Quan CHEN
1
;
Xue-Ying SHANG
;
Hua LI
;
Ren-An QIN
;
Feng-Lei HE
Author Information
1. 广州白云山陈李济药厂有限公司,广东广州 510288
- Keywords:
Guangdong Citri Reticulatae Pericarpium;
aging;
high performance liquid chromatography-diode array detector(HPLC-DAD);
characteristic spectrum;
Vicerenin-2;
cluster analysis;
quality control
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(8):2162-2168
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high performance liquid chromatography(HPLC)aging characteristic chromatogram of Guangdong Citri Reticulatae Pericarpium.Methods HPLC-diode array detector(DAD)was used to analyze the composition differences between different years of Guangdong Citri Reticulatae Pericarpium,and the relative peak area of each differential chromatographic peak was determined.The aging grade was classified by cluster analysis,and evaluated by the similarity evaluation system for chromatographic fingerprint of TCM(2012A edition).Results The different years of Guangdong Citri Reticulatae Pericarpium was clustered into two different aging grades of classⅠ and class Ⅱ.The similarity of class Ⅰ(low aging grade)Guangdong Citri Reticulatae Pericarpium was 0.708-0.957,and the similarity of class Ⅱ(high aging grade)Guangdong Citri Reticulatae Pericarpium was 0.734-0.976,and the similarity between the two was lower than 0.6.There were 9 different chromatographic peaks in the established characteristic chromatogram.The control characteristic spectrum T2 of typeⅡ Guangdong Citri Reticulatae Pericarpium can be regarded as its aging characteristic spectrum,among which peaks 1-4 and 9 are aging characteristic peaks.Conclusion The established aging characteristic chromatogram of Guangdong Citri Reticulatae Pericarpium is simple in operation,with good repeatability and reliability,and can be used for quality evaluation of different years of Guangdong Citri Reticulatae Pericarpium.
