Exploration of the Effect of Guhuaisi Kangfu Pills on Neovascularisation of Steroid-Induced Osteonecrosis of the Femoral Head in Rats Based on Gene Expression of VEGF/PI3K/Akt Pathway
10.13359/j.cnki.gzxbtcm.2024.08.028
- VernacularTitle:基于VEGF/PI3K/Akt通路基因表达探讨骨坏死康复丸对激素性股骨头坏死大鼠血管新生的影响
- Author:
Wen-Xi LI
1
;
Liang-Yu TIAN
;
Jin ZHANG
;
Cai-Hong SHEN
;
Zhi-Min YANG
;
Xiao-Yan FENG
;
Jia-Qiao GUO
;
Yu-Ju CAO
Author Information
1. 郑州中医骨伤病医院,河南郑州 450016;河南省骨坏死研究中医药重点实验室,河南郑州 450016;河南省骨伤病中医诊疗工程技术研究中心,河南郑州 450016
- Keywords:
Guhuaisi Kangfu Pills;
steroid-induced osteonecrosis of the femoral head(SONFH);
angiogenesis;
phosphatidylinositol 3-kinase(PI3K);
protein kinase B(Akt)1;
vascular endothelial growth factor(VEGF);
platelet endothelial cell adhesion molecule 1(CD31);
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(8):2127-2135
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the therapeutic effect and mechanism of Guhuaisi Kangfu Pills on rats with steroid-induced osteonecrosis of the femoral head(SONFH).Methods Sixty rats were randomly divided into blank group,model group,Xianling Gubao Capsules group and Guhuaisi Kangfu Pills low-,medium-and high-dose groups,10 rats in each group.Except for the blank group,the SONFH model was established by lipopolysaccharide combined with Glucocorticoid induction method in all other groups of rats.At the end of the intervention,for the femoral head,blood vessel radiography was performed to observe the microvascular changes in the bone marrow,and hematoxylin-eosin(HE)staining and calculation of the empty bone trap rate,Micro-CT scanning analysis,and compression experiments were carried out,and the real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the gene expressions of phosphatidylinositol 3-kinase(PI3K),protein kinase B(Akt)1,vascular endothelial growth factor(VEGF)and platelet endothelial cell adhesion molecule 1(CD31)in whole blood.Results Compared with the blank group,the blood supply in the femoral head medullary cavity of the model group was poor,the empty bone lacuna rate was increased(P＜0.05),the bone mineral density and bone volume fraction were significantly decreased(P＜0.05),the maximum load and elastic modulus of the femoral head were decreased(P＜0.05),and the mRNA expression levels of Akt1,PI3K,VEGF and CD31 in whole blood were decreased(P＜0.05).Compared with the model group,the blood supply in the femoral head medullary cavity was relatively good,the empty bone lacuna rate was decreased(P＜0.05),the bone mineral density,bone volume fraction,trabecular number and trabecular thickness were significantly increased(P＜0.05),the trabecular separation was significantly decreased(P＜0.05),the maximum load and elastic modulus of the femoral head were increased(P＜0.05),and the mRNA expression levels of Akt1,PI3K,VEGF and CD31 in the whole blood were increased(P＜0.05)in the high-dose group of Guhuaisi Kangfu Pills and Xianling Gubao Capsules group.There was no significant difference in the above indexes between the high-dose group of Guhuaisi Kangfu Pills and the Xianling Gubao Capsules group(P＞0.05).Conclusion Guhuaisi Kangfu Pills improves SONFH in rats,and its mechanism is related to the promotion of VEGF/PI3K/Akt pathway gene expression,thereby promoting angiogenesis.
