Effects of Astragalus Polysaccharide on the Development and Immune Escape of Non-Hodgkin's Lymphoma by Regulating Programmed Death Molecule 1/Programmed Death Ligand 1 Signaling Pathway
10.13359/j.cnki.gzxbtcm.2024.07.031
- VernacularTitle:黄芪多糖调节程序性死亡分子1/程序性死亡配体1信号通路对非霍奇金淋巴瘤发展和免疫逃逸的影响
- Author:
Jiao-Min CHEN
1
;
Wei-Xing XU
;
Wei ZHANG
Author Information
1. 沧州市中心医院血液内一科,河北沧州 061001
- Keywords:
astragalus polysaccharide;
non-Hodgkin's lymphoma;
immune escape;
programmed death molecule 1(PD-1);
programmed death ligand 1(PD-L1);
BJAB cells;
SP53 cells;
WSU-DLCL2 cells
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(7):1865-1872
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the therapeutic effect and mechanism of astragalus polysaccharide on non-Hodgkin's lymphoma(NHL).Methods Three kinds of NHL cells,WSU-DLCL2,BJAB and SP53,were cultured and treated with different concentrations(0,5,10,50,100,200,400 μmol/L)of astragalus polysaccharide.The cell viability was detected by cell counting kit-8(CCK-8)method to screen the optimal concentration of astragalus polysaccharide and the cells sensitive to astragalus polysaccharide.WSU-DLCL2 cells were divided into control group,astragalus polysaccharide group,BMS-1[programmed death molecule 1(PD-1)/programmed death ligand 1(PD-L1)pathway inhibitor]group,astragalus polysaccharide+BMS-1 group.The colony formation,apoptosis,migration and invasion of each group were detected,and the protein expression of PD-1/PD-L1 signaling pathway was detected by Western Blot.After co-culture of WSU-DLCL2 cells and CD8+T cells,the percentage and apoptosis of CD8+T cells,and the levels of interferon(IFN)-γ and tumor necrosis factor(TNF)-α in the supernatant were detected.Results The follow-up experiments selected 200 μmol/L astragaloside to treat WSU-DLCL2 cells.Compared with the control group,the number of WSU-DLCL2 cell colony formation,the number of migrating WSU-DLCL2 cells,the number of invading WSU-DLCL2 cells,the apoptosis rate of CD8+T cells,and the protein expression levels of PD-1 and PD-L1 in WSU-DLCL2 cells were significantly decreased in the Astragali polysaccharide group,the BMS-1 group and the Astragali polysaccharide+BMS-1 group(P＜0.05),and the apoptosis rate of WSU-DLCL2 cells,the proportion of CD8+T cells,and the levels of IFN-γ and TNF-α in the supernatant of co-culture system were significantly increased(P＜0.05);compared with astragali polysaccharide group and BMS-1 group,the number of WSU-DLCL2 cell colony formation,migrating WSU-DLCL2 cells,invading WSU-DLCL2 cells,CD8+T-cell apoptosis rate,and protein expressions of PD-1,PD-L1 in WSU-DLCL2 cells were further decreased in astragali polysaccharide+BMS-1 group(P＜0.05),and the rate of WSU-DLCL2 cell apoptosis,the proportion of CD8+T-cells,and the levels of IFN-γ,TNF-α in the supernatant of co-culture system were significantly further elevated(P＜0.05).Conclusion Astragalus polysaccharide can inhibit NHL cell proliferation,migration and invasion,promote its apoptosis and reduce immune escape,and its mechanism may be related to the inhibition of PD-1/PD-L1 signaling pathway.
