Mechanism of Carthami Flos- Paeoniae Raidx Alba herb pair in treatment of migraine based on network pharmacology and experimental verification
10.3760/cma.j.cn121382-20240129-00308
- VernacularTitle:基于网络药理学及实验验证探讨红花-白芍药对治疗偏头痛的作用机制
- Author:
Xiaolin TIAN
1
;
Na XU
Author Information
1. 天津医科大学第二医院康复医学科，天津 300211
- Keywords:
Migraine;
Carthami Flos- Paeoniae Raidx Alba herb pair;
Network pharmacology;
Mechanism
- From:
International Journal of Biomedical Engineering
2024;47(3):255-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanism of Carthami Flos- Paeoniae Raidx Alba herb pair in the treatment of migraine based on network pharmacology and experimental verification. Methods:TCMSP was queried to screen the drug components of Carthami Flos and Paeoniae Raidx Alba, and Swiss Target Prediction was used to predict the drug components. GeneCards was conducted to obtain disease targets for migraine, and Venny 2.1 was used to obtain intersection targets. PPI analysis with STRING and network diagrams with Cytoscape, GO and KEGG analysis with Metascape. The "drug-target-pathway" network was constructed by Cytoscape software. The migraine rat model was established by the nitroglycerin method, and the rats were divided into a model group and an observation group (5 rats in each group) according to the random number table method. The observation group was given 1.8 g/kg Carthami Flos- Paeoniae Raidx Alba solution, while the model group was given 0.9% sodium chloride solution, once a day, for 2 weeks. Symptom improvement was evaluated after treatment, and the core target of serum V-Rel endotheloproliferative virus oncogene homology A (RELA), protein kinase B1 (Akt1), mitogen-activated protein kinase 1 (MAPK1), tumor necrosis factor (TNF), cysteine aspartic protease-3 (Caspase-3), and interleukin-6 (IL-6) expression was detected. Results:It was found that 24 active ingredients in Carthami Flos- Paeoniae Raidx Alba herb pair directly act on 178 disease targets through multiple pathways to treat migraine. Among them, 6-hydroxykaempferol, beta-carotene, baicalin, beta-sitosterol and kaempferol were the core components. RELA, Akt1, MAPK1, TNF, Caspase-3, IL-6 were critical targets. The GO analysis showed 85 cellular component (CC), 228 biological process (BP) and 119 molecular function (MF). Enrichment analysis of KEGG signaling pathway suggested that Carthami Flos- Paeoniae Raidx Alba herb pair was mainly involved in advanced glycosylated end-product (AGE)-advanced glycosylated end-product receptor (RAGE), hypoxia-inducing factor-1 (HIF-1), phosphatidylinositol 3-kinase (PI3K)-protein kinase B (Akt) and MAPK signaling pathways. The experimental results showed that compared with the model group, the scores of Carthami Flos- Paeoniae Raidx Alba herb pair decreased at 0 ~ 30, 60 ~ 90, and 120 ~ 150 min (all P < 0.01), and the pain threshold of mechanical and thermal stimuli increased (all P < 0.01). Serum levels of RELA, Akt1, MAPK1, TNF, Caspase-3, and IL-6 were decreased (all P < 0.01). Conclusions:Carthami Flos- Paeoniae Raidx Alba herb pair can treat migraine by regulating RELA, Akt1, MAPK1, TNF, Caspase-3 and IL-6 signaling pathways including IL-17, TNF, Toll receptor, T cell receptor, AGE-RAGE, HIF-1, PI3K/Akt and MAPK.
