Relationship between SERPINE1 gene related to lactic acid metabolism and clinical prognosis of gastric cancer patients
10.3969/j.issn.1673-4130.2024.21.010
- VernacularTitle:乳酸代谢相关的SERPINE1基因与胃癌患者临床预后的关系
- Author:
Kai SUN
1
;
Xiaodong WANG
;
Yibing ZHOU
Author Information
1. 佳木斯市中心医院普外二科,黑龙江佳木斯 154002
- Keywords:
gastric cancer;
lactic acid;
clinical prognosis;
serum
- From:
International Journal of Laboratory Medicine
2024;45(21):2610-2614,2619
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between SERPINE1 gene related to lactic acid metabo-lism and clinical prognosis of gastric cancer(GC).Methods A total of 89 patients with GC who underwent surgical resection in the hospital from January 2018 to December 2020 were enrolled in the study.In the pre-liminary experiment,the levels of 5 genes related to lactic acid metabolism(F5,MTTP,SERPINE1,CYP19A1,SLC52A3)in 24 serum samples from GC patients with stage Ⅰ(n=12)and stage Ⅳ(n=12)were analyzed.In the second step,the candidate genes with significant changes in serum of GC patients with stage Ⅳ and GC patients with stage Ⅰ in the preliminary experiment were verified in a larger independent co-hort,which included serum samples of 65 GC patients and 22 healthy controls.Results Among the genes re-lated to lactic acid metabolism(F5,MTTP,SERPINE1,CYP19A1,SLC52A3)analyzed in the preliminary ex-periment,SERPINE1 was significantly up-regulated in the serum of patients with stage Ⅳ compared with that in the serum of patients with stage Ⅰ(P＜0.001).The expression level of SERPINE1 in serum of GC pa-tients was significantly lower than that of healthy controls(P＜0.000 1).In addition,the expression level of SERPINE1 increased with the progress of TNM staging(P＜0.05).Based on the receiver operating charac-teristic curve,the maximum predictive value of SERPINE1 on overall survival(OS)rate was analyzed(area under the curve=0.725,cut off value=0.002 1),and GC patients were divided into high expression group(n=39)or low expression group(n=26).Compared with the SERPINE1 low expression group,the cases of venous infiltration,T3/T4 stage,lymph node metastasis and distant metastasis in the SERPINE1 high expres-sion group increased,and the differences were statistically significant(P＜0.05).The OS rate of GC patients with low expression of SERPINE1 was significantly higher than that of patients with high expression of SER-PINE1(P＜0.05).Multivariate analysis showed that the high expression of SERPINE1 in serum(HR=4.51,95％CI=1.23-23.69,P=0.021)was an independent prognostic marker for predicting the poor prog-nosis of GC patients.Conclusion SERPINE1 gene related to lactic acid metabolism in serum could be used as a reliable biomarker for the prognosis of GC.
