Expression and significance of hsa_circ_0082002 in serum of patients with primary hepatocellular carcinoma
10.3969/j.issn.1673-4130.2024.20.016
- VernacularTitle:hsa_circ_0082002在原发性肝细胞癌患者血清中的表达及意义
- Author:
Qinjun WANG
1
;
Shenjie JI
;
Nan HUANG
;
Ying HUANG
Author Information
1. 启东市人民医院/启东肝癌防治研究所/南通大学附属启东医院检验科,江苏启东 226200
- Keywords:
primary hepatocellular carcinoma;
circular RNA;
diagnostic value
- From:
International Journal of Laboratory Medicine
2024;45(20):2511-2514,2519
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression level of hsa_circ_0082002 in serum of patients with primary hepatocellular carcinoma(HCC)and explore its auxiliary diagnostic value.Methods A total of 70 HCC pa-tients treated in this hospital from January 2020 to December 2022 were selected as the HCC group,55 pa-tients with liver cirrhosis(LC)were selected as the LC group,55 patients with chronic hepatitis B(CHB)were selected as the CHB group,and 50 healthy people were selected as the control group.The relative expres-sion level of hsa_circ_0082002 in serum of each group was detected by real-time fluorescent quantitative PCR,and the level of serum alpha-fetoprotein(AFP)was detected by electrochemiluminescence.The receiver oper-ating characteristic(ROC)curve was used to evaluate the differential diagnostic efficacy of hsa_circ_0082002 and AFP for HCC.Results The relative expression levels of serum hsa_circ_0082002 in HCC group,LC group,CHB group and control group were 2.750(1.925,4.393),2.199(1.416,2.850),1.591(0.925,2.107)and 1.035(0.666,1.680),respectively.The relative expression level of serum hsa_circ_0082002 in the HCC group was higher than those in the LC group,CHB group and HC group,and the differences were statistically signifi-cant(all P＜0.05).The results of ROC curve showed that the area under the curve(AUC)of hsa_circ_0082002 and AFP in the differential diagnosis of HCC were 0.813 and 0.779,respectively.The AUC of combined detection of hsa_circ_0082002 and AFP was 0.871,with a sensitivity of 75.71％and specificity of 83.13％.Conclusion Serum hsa_circ_0082002 may be a biomarker for the diagnosis of HCC.
