Analysis of the value of fecal miR-92a combined with serum CEA,CA199 and VEGF detection in the diagnosis of colon cancer
10.3969/j.issn.1673-4130.2024.19.017
- VernacularTitle:粪便miR-92a联合血清CEA、CA199、VEGF检测在结肠癌诊断中的价值分析
- Author:
Jiankang CHEN
1
;
Jie MIAO
Author Information
1. 空军军医大学第一附属医院检验科,陕西西安 710032
- Keywords:
microRNA-92a;
carcinoembryonic antigen;
carbohydrate antigen 199;
vascular endothe-lial growth factor;
colon cancer
- From:
International Journal of Laboratory Medicine
2024;45(19):2392-2395
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diagnostic value of fecal microRNA-92a(miR-92a)and serum car-cinoembryonic antigen(CEA),carbohydrate antigen 199(CA199)and vascular endothelial growth factor(VEGF)in patients with colon cancer.Methods A total of 77 patients with colon cancer admitted to the First Affiliated Hospital of Air Force Medical University from January 2020 to August 2022 were selected as the co-lon cancer group,including 19 patients who underwent laparoscopic radical resection of colon cancer and were followed up for 6 months after surgery.Another 70 healthy subjects were selected as the control group and 81 patients with other digestive system tumors were selected as the other digestive system tumors group.Real-time fluorescence quantitative PCR was used to detect the level of fecal miR-92a,electrochemiluminescence was used to detect the levels of serum CEA and CA199,and chemiluminescence was used to detect the level of serum VEGF.The receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of miR-92a,CEA,CA199 and VEGF in colon cancer.Results The positive rate of fecal miR-92a in colon cancer group was significantly higher than that in other digestive system tumors group and control group(P＜0.001).The serum levels of CEA,CA199 and VEGF in colon cancer group were significantly higher than those in control group(P＜0.05).The positive rate of miR-92a and the levels of serum CEA,CA199 and VEGF in colon cancer patients were significantly lower at 6 months after surgery than those before surgery(P＜0.05).ROC curve analysis showed that the combined detection of CEA,CA199,VEGF and miR-92a had the largest area under the curve(AUC,0.901),sensitivity(77.9％)and specificity(91.3％).When miR-92a+CEA was combined,the AUC was 0.899,the sensitivity was 77.9％,and the specificity was 92.8％.Conclusion The detection of miR-92a,CEA,CA199 and VEGF has important value in the diagnosis of colon cancer.The combined detection of miR-92a and CEA can improve the sensitivity and specificity of the diagno-sis of colon cancer,and has a certain value in the prognosis of patients with colon cancer.
