Predictive value of DNA methylation degree of serum Wnt4 gene promoter region in preterm patients
10.3969/j.issn.1673-4130.2024.13.009
- VernacularTitle:血清Wnt4基因启动子区的DNA甲基化程度对早产患者预测价值分析
- Author:
Lan QIU
1
;
Qiongying XIONG
;
Dafen CAI
Author Information
1. 武汉科技大学附属孝感医院妇产科,湖北孝感 432000
- Keywords:
Wnt4 gene;
DNA methylation degree;
premature delivery;
prediction
- From:
International Journal of Laboratory Medicine
2024;45(13):1580-1585
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the predictive value of DNA methylation degree of serum Wnt4 gene pro-moter region in preterm patients.Methods A total of 480 pregnant women were randomly selected from the obstetrics ward of the hospital from January to December 2019.Among them,450 were followed up until de-livery,and 40 spontaneous preterm pregnant women were selected as the preterm group.410 full-term preg-nant women were selected as the health group.The DNA methylation degree of serum Wnt4 gene promoter region was compared between health group and preterm group,and the pregnant women with different gesta-tional week in preterm group.The clinical data of health group and preterm group were compared.Logistic re-gression analysis was used to analyze the influencing factors of premature delivery.Receiver operating charac-teristic(ROC)curve was plotted to analyze the predictive value of DNA methylation degree of serum Wnt4 gene promoter region for premature delivery.Results Compared with health group,the DNA methylation de-gree of serum Wnt4 gene promoter region in preterm group was significantly increased(P<0.05).Compared with pregnant women with 34-<37 gestational weeks in the preterm group,the DNA methylation degree of serum Wnt4 gene promoter region in pregnant women with 28-<34 gestational weeks was significantly in-creased(P<0.05).Compared with the health group,the proportions of pregnancies≥2 times,passive smok-ing during pregnancy,positive vaginal fetal fibronectin(fFN),and average monthly household income<1 000 yuan in the preterm group were significantly increased(P<0.05),and cervical length was significantly short-ened(P<0.05).Logistic regression analysis showed that pregnancies≥2 times,passive smoking during pregnancy,short cervical length,positive vaginal fFN,average monthly household income<1 000 yuan and high degree of DNA methylation in serum Wnt4 gene promoter region were all risk factors for premature de-livery(P<0.05).ROC curve results showed that the area under the curve of DNA methylation degree of ser-um Wnt4 gene promoter region for predicting premature delivery was 0.868(95%CI:0.834-0.898),the cut-off value was 51.43%,and sensitivity,specificity and accuracy were 82.50%,84.63%and 84.44%,respective-ly.Conclusion The high degree of DNA methylation in serum Wnt4 gene promoter region has certain clinical value in predicting the premature delivery.
