Plasma CircOGDH,CircHECTD1 expression in patients with acute ischemic stroke and their clinical significance
10.3969/j.issn.1673-4130.2024.13.005
- VernacularTitle:急性缺血性脑卒中患者血浆CircOGDH、CircHECTD1表达及临床意义
- Author:
Yan LI
1
;
Yao WANG
;
Dongmei WANG
Author Information
1. 哈尔滨市中医院检验科,黑龙江哈尔滨 150010
- Keywords:
acute ischemic stroke;
circular RNA OGDH;
circular RNA HECTD1;
vascular cognitive impairment;
prognosis
- From:
International Journal of Laboratory Medicine
2024;45(13):1558-1563
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the plasma cyclic RNA(Circ)OGDH,CircHECTD1 expression in pa-tients with acute ischemic stroke(AIS)and their clinical significance.Methods A total of 112 AIS patients admitted to the hospital from December 2020 to December 2022 were selected as the AIS group,while 60 healthy individuals who underwent physical examinations during the same period were selected as the control group.Based on whether they had vascular cognitive impairment(VCI)at the 3-month follow-up after dis-charge,AIS patients were divided into VCI group(60 cases)and non VCI group(52 cases).According to the prognosis of AIS patients at the 3-month follow-up after discharge,they were divided into good prognosis group(87 cases)and poor prognosis group(25 cases).Real-time fluorescence quantitative PCR was used to detect the plasma expression of CircOGDH and CircHECTD1.The Simple Intelligent Mental Scale(MMSE),Montreal Cognitive Assessment Scale(MoCA),and National Institutes of Health Stroke Scale(NIHSS)scores were used to assess the degree of VCI and neurological impairment in AIS patients.Pearson correlation analysis was conducted to analyze the correlation between CircOGDH,CircHECTD1 and clinical parameters.Factors affecting the poor prognosis of AIS patients were analyzed by Multivariate Logistic regression analy-sis.Receiver operating characteristic(ROC)curve was used to analyze the evaluation value of CircOGDH and CircHECTD1 for poor prognosis in AIS patients.Results The plasma relative expression levels of CircOGDH and CircHECTD1 in the AIS group were higher than those in the control group(t=23.616,29.181,P<0.05).The plasma relative expression levels of CircOGDH and CircHECTD1 in the VCI group were higher than those in the non VCI group(t=12.657,27.667,P<0.05).Compared with the good prognosis group,the poor prognosis group had greater infarct size,higher 24 h NIHSS score at admission,and higher relative ex-pression levels of CircOGDH,and CircHECTD1,while the MMSE score and MoCA score at 3 months after discharge were significantly lower(P<0.05).The plasma relative expression levels of CircOGDH and Cir-cHECTD1 in AIS patients were significantly positively correlated with infarct size and 24 h NIHSS score at admission,while they were significantly negatively correlated with MMSE score and MoCA score at 3 months after discharge(P<0.05).Elevated relative expression levels of CircOGDH,CircHECTD1,larger infarct size,and higher 24 h NIHSS score at admission were independent risk factors for poor prognosis in AIS pa-tients(all P<0.05).The area under the curve of the combination of plasma CircOGDH and CircHECTD1 for predicting the poor prognosis in AIS was 0.903(95%CI:0.852-0.955),which was significantly larger than 0.831(95%CI:0.781-0.866)and 0.836(95%CI:0.794-0.870)of the single indicator(Z=2.258,2.119,P=0.021,0.031).Conclusion The plasma CircOGDH and CircHECTD1 expression in AIS patients is up-regulated,which is related to the degree of VCI and neurological impairment in patients.The combination of these two factors has high predictive value for the poor prognosis in AIS patients.
