Application of Protease-Hydrogen Peroxide Digestion Method in Forensic Diatom Examination
10.12116/j.issn.1004-5619.2024.440116
- VernacularTitle:蛋白酶-过氧化氢消解法在法医学硅藻检验中的应用
- Author:
Wen-Qing GUO
1
;
Min CHEN
;
Ao MA
;
Ping HUANG
;
Ji ZHANG
Author Information
1. 山西医科大学法医学院,山西 太原 030001;司法鉴定科学研究院 上海市法医学重点实验室 司法部司法鉴定重点实验室 上海市司法鉴定专业技术服务平台,上海 200063
- Keywords:
forensic pathology;
death from drowning;
diatom examination;
protease-hydrogen peroxide digestion method
- From:
Journal of Forensic Medicine
2024;40(4):317-323
- CountryChina
- Language:Chinese
-
Abstract:
Objective To propose a novel forensic diatom examination method,namely the protease-hydrogen peroxide(PHP)digestion method,and to explore its potential application in forensic prac-tice.Methods The PHP digestion method was applied to process lung tissues and compared with the traditional nitric acid digestion method in terms of diatom quantity,recovery rate,proportion of diatom fragments,and recovery efficiency of different diatom genera.Results The PHP digestion method showed higher diatom quantity,recovery rate,and proportion of diatom fragments compared to the tra-ditional nitric acid digestion method with statistical significance.The average diatom recovery rate was as high as 93.95%.In the lung tissues digested with nitric acid,there were 65 Achnanthes,36 NitzschiaⅠ,22 Nitzschia Ⅱ,20 Diploneis and 8 Cymbella,showing significantly lower recovery rates compared to water samples which had 154 Achnanthes,44 Nitzschia Ⅰ,45 Nitzschia Ⅱ,33 Diploneis and 23 Cym-bella.However,with the PHP digestion method,only Nitzschia Ⅱ showed a lower recovery rate com-pared to water samples(P<0.05).At the same time,the diatom recovery stability of the PHP diges-tion method was higher than that of the nitric acid digestion method.Conclusion The PHP digestion method is superior to the traditional nitric acid digestion method in the lung tissue diatom extraction efficiency.Additionally,it holds significant advantages in safety,environmental protection,and other as-pects.It is expected to be applied in forensic drowning identification.
