Rapid Determination of Paraquat and Diquat Residues in Tea by QuEChERS/Ultra-High Performance Liquid Chromatography-Tandem Quadrupole Mass Spectrometry
10.19756/j.issn.0253-3820.241265
- VernacularTitle:改进QuEChERS/超高效液相色谱-串联三重四极杆质谱法快速检测茶叶中百草枯和敌草快残留
- Author:
Yan-Hua TANG
1
;
Ying-Nan GAO
;
Jiang-Yan CHEN
;
Xin-Yi GUO
;
Shao-Yong LI
;
Yue LI
;
Zhen ZHOU
;
Wei GAO
Author Information
1. 暨南大学环境与气候学院,质谱仪器与大气环境研究所,广州 510632
- Keywords:
QuEChERS;
Ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry;
Tea;
Paraquat;
Diquat
- From:
Chinese Journal of Analytical Chemistry
2024;52(10):1619-1627
- CountryChina
- Language:Chinese
-
Abstract:
A rapid analytical method for determination of paraquat and diquat in tea samples was established by improving the QuEChERS pre-treatment method combined with ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry(UPLC-MS/MS).Four kinds of tea powder were extracted by using acetonitrile-0.1％formic acid solution(3∶7,V/V)and ultrasonic treatment,and the supernatant was purified with 400 mg C18 and 400 mg PSA and separated on hydrophilic HILIC(100 mm×2.1 mm,1.8 μm)column by using 0.1％formic acid aqueous solution(Containing 5 mmol/L ammonium formate)and acetonitrile as mobile phases.In the electrospray ion source positive ion mode(ESI+),multiple reaction monitoring scanning technology(MRM)was used for determination,and the matrix-matched standard solution external standard method was used for quantitative analysis.The results showed that paraquat obtained good linear relationship in the content range of 0.18-200 μg/kg and diquat obtained good linear relationship in the content range of 0.36-200 μg/kg,and the correlation coefficients(R2)were 0.9939-0.9976 and 0.9959-0.9987,respectively.The limits of detection(LODs)were 0.06 and 0.12 μg/kg,and the limits of quantitation(LOQs)were 0.18 and 0.36 μg/kg,respectively.The average spiked recoveries for tea samples varied from 84.4％to 128.8％,with the relative standard deviations(RSDs)from 0.6％to 13.5％.The method was simple and efficient,with accurate quantification ability and low detection limit,which could realize efficient determination of paraquat and diquat in tea samples.
