ZnO Quantum Dot and SnO2 Quantum Dot Labeling Antibodies and Single Particle Inductively Coupled Plasma Mass Spectrometry Coupled with Magnetic Immunoassay for Detection of Ovarian Cancer Biomarkers CA125 and HE4 in Human Serum
10.19756/j.issn.0253-3820.241206
- VernacularTitle:ZnO量子点和SnO2量子点标记抗体结合磁免疫单颗粒电感耦合等离子体质谱法测定人血清中卵巢癌生物标志物CA125和HE4
- Author:
Yu-Pin CAO
1
;
Hai-Ping CHEN
;
Jie LIANG
;
Bi-Yang DENG
Author Information
1. 广西中医药大学药学院,广西高校中药提取纯化与质量分析重点实验室,南宁 530200
- Keywords:
Zinc oxide quantum dot;
Tin oxide quantum dot;
Carbohydrate antigen 125;
Human epididymis protein 4;
Inductively coupled plasma mass spectrometry;
Single particle analysis
- From:
Chinese Journal of Analytical Chemistry
2024;52(10):1562-1572
- CountryChina
- Language:Chinese
-
Abstract:
Ovarian cancer is one of the most fatal of all the gynecological malignancies.The detection of biomarker is one of the diagnostic bases for the disease.The combined detection of carbohydrate antigen 125(CA125)and human epididymis protein 4(HE4)in human serum can improve the accuracy of clinical diagnosis for ovarian cancer.In this study,a new method for analysis of ovarian cancer biomarkers CA125 and HE4 in human serum using zinc oxide quantum dot(ZnO QD)and tin oxide quantum dot(SnO2 QD)labeled antibodies coupled with single particle inductively coupled plasma mass spectrometry(ICP-MS)was developed.CA125 and HE4 antigens were captured by amino-modified magnetic nanoparticles combined with CA125 and HE4 primary antibodies.CA125 and HE4 secondary antibodies were labeled by ZnO QD and SnO2 QD,respectively.The signal frequency of Zn and Sn was determined by ICP-MS to realize the quantitative analysis of CA125 and HE4.The linear detection ranges of CA125 and HE4 were 0.02-200 U/mL and 0.02-100 ng/mL,the detection limits were 0.004 U/mL and 0.006 ng/mL(3σ),and the RSDs were 2.2％and 3.5％(n=6),respectively.This method had many advantages such as high sensitivity,good selectivity and less reagent consumption,and was successfully applied to determine the content of CA125 and HE4 in human serum.
