Investigation of Nephrotoxicity Induced by Mecury(Ⅱ)Exposure by Mass Spectrometry Imaging
10.19756/j.issn.0253-3820.241249
- VernacularTitle:基于质谱成像技术解析无机汞暴露致肾脏毒性的特征
- Author:
Chang LIU
1
;
Ming XU
Author Information
1. 中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京 100085;国科大杭州高等研究院环境学院,杭州 310024;中国科学院大学资源与环境学院,北京 100049
- Keywords:
Inorganic mercury;
Nephrotoxicity;
Mass spectrometry imaging;
Metabolite;
Toxicological mechanism
- From:
Chinese Journal of Analytical Chemistry
2024;52(10):1553-1561,中插25-中插28
- CountryChina
- Language:Chinese
-
Abstract:
The kidneys are the primary organ for the accumulation of inorganic mercury(Hg(Ⅱ)),which can lead to severe renal injuries.Nevertheless,the current understanding of Hg(Ⅱ)-induced nephrotoxicity is restricted to biochemical and histological methods,which might not fully identify the features of renal damages.In this study,we aimed to elucidate the characteristics and mechanisms of Hg(Ⅱ)-induced nephrotoxicity using air flow-assisted desorption electrospray ionization mass spectrometry imaging(AFADESI-MSI),which enabled us to examine the injury and key metabolite profile in the kidneys of mice exposed to Hg(Ⅱ)in drinking water.Histopathological analysis result showed that following a 28-day exposure of 3 mg/L Hg(Ⅱ),the glomeruli appeared unaffected,whilst the renal tubular lumen showed slight dilation,the tubular epithelial cells displayed flattening,and there was moderate interstitial congestion accompanied by lymphocyte infiltration.More importantly,the results showed that MSI offered a more sensitive detection of biomarkers linked to kidney damages,like creatinine,than blood biochemical assay frequently used for renal function assessment.This made MSI a valuable tool for toxicity assessment and disease diagnosis because it enabled the early recognition of kidney injury at the molecular level.Meanwhile,the results revealed that Hg(Ⅱ)exposure resulte d in elevation of spermidine,arginine,creatine,and creatinine level in cortex,medullaria,and renal pelvis.Additionally,Hg(Ⅱ)also led to increase of reduced glutathione,oxidized glutathione,and cysteine level in these regions.All these findings illustrated that Hg(Ⅱ)could interfere with urea cycle and caused aberrant kidney function by disrupting the redox homeostasis.In conclusion,MSI would play an important role on the health risks and toxicological mechanisms of heavy metals in the future.
