Advances in Inductively Coupled Plasma-Mass Spectrometry for Detection of Endogenous and Exogenous Substances in Single Cells
10.19756/j.issn.0253-3820.241223
- VernacularTitle:电感耦合等离子体质谱技术检测单细胞内外源物质的研究进展
- Author:
Tao XU
1
;
Xiang-Wei TIAN
;
Yan-Wei LIU
;
Ying-Ying GUO
;
Li-Gang HU
;
Yong-Guang YIN
;
Qing-Hua ZHANG
;
Yong CAI
;
Gui-Bin JIANG
Author Information
1. 中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京 100085;国科大杭州高等研究院环境学院,杭州 310024;中国科学院大学,北京 100049
- Keywords:
Inductively coupled plasma-mass spectrometry;
Single-cell analysis;
Metals;
Exogenous substances;
Endogenous substances;
Review
- From:
Chinese Journal of Analytical Chemistry
2024;52(10):1403-1412,中插1-中插9
- CountryChina
- Language:Chinese
-
Abstract:
Cells are the fundamental structural and functional units of biological organisms,with inherent differences in composition and interactions with exogenous substances,known as cellular heterogeneity.Single cell inductively coupled plasma-mass spectrometry(SC-ICP-MS)allows for the high-throughput introduction of individual cells,enabling the highly sensitive detection and quantification of elements within a single cell,thus effectively providing information on cellular heterogeneity.This review outlined the SC-ICP-MS sample preparation process for different types of cells(single-cell systems,aggregation-prone and adherent cell systems,animal tissues,and plant tissues),including steps such as separation,washing,and fixation,as well as the advantages and existing issues of the current sample introduction systems and quantification methods.The recent applications of SC-ICP-MS in detecting endogenous substances(endogenous elements and proteins),exogenous substances(heavy metals,metal-based drugs and nanoparticles),and the simultaneous detection of both endogenous and exogenous substances were summarized.Finally,the perspectives on the future development of SC-ICP-MS in analytical methods and application fields were presented,including the optimization of single-cell sample preparation,transport efficiency,evaluation standards of ionization efficiency,and the establishment of multiparametric cell analysis platforms.
