Determination and Ecological Risk Assessment of Sulfonamides in Environment Water by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
10.19756/j.issn.0253-3820.231394
- VernacularTitle:水环境中磺胺类抗生素的超高效液相色谱-串联质谱法测定及其生态风险评估
- Author:
Wen-Yan WU
1
;
Hong-Mei HU
;
Peng-Fei HE
;
Yi FANG
;
Tie-Jun LI
;
Yuan-Ming GUO
Author Information
1. 浙江省海洋水产研究所,浙江省海洋渔业资源可持续利用技术研究重点实验室,舟山 316021;上海交通大学环境科学与工程学院,上海 200240
- Keywords:
Sulfonamides antibiotics;
Automated solid-phase disk extraction;
Ultra high performance liquid chromatography-tandem mass spectrometry;
Water environment;
Ecological risk
- From:
Chinese Journal of Analytical Chemistry
2024;52(8):1182-1191,中插12-中插20
- CountryChina
- Language:Chinese
-
Abstract:
The abuse and irrational use of antibiotics in human veterinary medicine has seriously endangered the ecological environment and human health.In this study,a fully automatic solid-phase disk extraction-stable isotope dilution-ultra-high performance liquid chromatography-mass spectrometry method for determination of 17 kinds of sulfonamides antibiotics(SAs)in water was established,which was then applied to determination of SAs in real samples including tap water,river water and seawater,respectively.Meanwhile,the residual characteristics were discussed and the ecological risks were assessed.With this method,1.0 L water sample with 0.5 g/L Na2EDTA(pH=3)was extracted and enriched by 3M SDB-XC disk,and eluted by 10 mL of mixture of methanol and acetone(1:1,V/V),and the pretreatment time of the sample was about 60 min per six samples.Under the optimized conditions,the linearity of the method for detection of 17 kinds of SAs ranged from 0.05 to 100 μg/L,with correlation coefficients(R2)>0.999.In addition,the detection limits(S/N=3)were as low as 0.012-0.052 ng/L,and the recoveries were in the range of 76%-110%,with relative standard deviations of 0.5%-9.6%(n=5).The results showed that no SAs was detected in tap water,while 3 and 9 kinds of SAs were detected in river water of Zhoushan,and seawater of Wenzhou Sea area in Zhejiang province,respectively.The total concentrations of the detected SAs were 0.875-21.826 ng/L and 1.024-20.768 ng/L in river water and seawater,respectively,and among which,sulfamethoxazole(SMX)was the predominant compound in river water and seawater,accounting for 81%and 74%of the total SAs,respectively.The ecological risk assessment showed that the risk quotients of the detected SAs in the river water and seawater in the study area for the three kinds of trophic organisms(algae,Daphnia and fish)were far less than 0.01,meaning that the ecological risk was low.
