Progress on Application of Magnetic Particles in Biomedical Assays Including Detection of Circulating Tumor Cell and Virus,Extraction and Detection of Nucleic Acids
10.19756/j.issn.0253-3820.231320
- VernacularTitle:磁性微球在循环肿瘤细胞和病毒以及核酸分离富集和检测分析中的应用进展
- Author:
Ling-Xiang GUO
1
;
Jian-Ping ZHOU
;
Tao YANG
Author Information
1. 东南大学 化学化工学院,南京211189
- Keywords:
Magnetic particles;
Circulating tumor cell;
Virus antigen;
Nucleic acids detection;
Review
- From:
Chinese Journal of Analytical Chemistry
2024;52(6):778-788
- CountryChina
- Language:Chinese
-
Abstract:
Magnetic beads (MB),composed of magnetic nanoparticles and biocompatible materials including cellulose,chitosan,silica and polystyrene,are ideal platforms for specific detection of cancer cells,virus and nucleic acids when loaded with antibodies,fluorescent and conjugation molecules. With the ease of separations using magnets,magnetic particles show tremendous advantages over other platforms and thus arising potentials in circulating tumor cell (CTC),virus (such as SARS-CoV-2) antibody and antigen detections,and nucleic acid extractions and purifications. In addition,when combined with microfluidic devices,magnetic particles can be used to develop detection platforms with high throughputs,as well as portable and point-of-care apparatus. In accompany with their huge potentials in all kinds of biomedical and clinical scenarios,magnetic particles based medical devices have been commercialized such as antigen test kits that have been commonly used during the pandemic. In this review,the progress in detailed antibody conjugation practice used in each application scenario based on MB platform was summarized and the commercialization of analytical devices combined with microfluidics and others was discussed.
