Preparation of Polyethylenimine Modified Nickel Ferrite-Graphene Oxide Material and Its Application in Enrichment of Phosphopeptides
10.19756/j.issn.0253-3820.231374
- VernacularTitle:聚乙烯亚胺修饰的铁酸镍-氧化石墨烯复合物在磷酸化多肽富集中的应用
- Author:
Kai-Na ZHANG
1
;
Yuan LIU
;
Xiang-Feng WANG
;
Hai-Ling LIU
;
Meng-Xia XIE
Author Information
1. 北京师范大学分析测试中心,北京 100875;中国农业大学生物学院,植物抗逆高效全国重点实验室,北京 100193
- Keywords:
Phosphopeptides;
Nickel ferrite;
Polyethylenimine;
Graphene oxide;
Magnetic response
- From:
Chinese Journal of Analytical Chemistry
2024;52(5):664-673
- CountryChina
- Language:Chinese
-
Abstract:
The NiFe2O4-graphene oxide nanocomposite modified with polyethylenimine(GO@PEI-NiFe2O4)was prepared to purify and enrich phosphopeptides from biosamples.The Ni2+and Fe3+ions on its surface could coordinate with phosphate groups and then selectively adsorb phosphopeptides.PEI was conducive to the above combination due to its high hydrophilicity.The material showed good magnetic response properties and could be rapidly separated from samples with the aid of magnet.With tryptic digest of β-casein as sample,the enrichment property of the material to phosphopeptides was studied,which was compared with the results of GO@NiFe2O4,revealing the adsorption mechanism of GO@PEI-NiFe2O4.The static and dynamic binding properties of GO@PEI-NiFe2O4 were investigated using pTyr as a representative phosphopeptide,and the adsorption capacity was 36.2 μg/mg.The results showed that the material could remove the interference of nonphosphopeptides and effectively enrich phosphopeptides in complex matrix.After enrichment by GO@PEI-NiFe2O4,1535 phosphopeptides were identified from the tryptic digest of rat liver by mass spectrum and the enrichment effect of GO@PEI-NiFe2O4 greatly outperformed commercial Fe3+-IMAC kits.This work provided an efficient material for the enrichment of phosphopeptides,showing potential applications in phosphoproteomics research.
