Advances of CRISPR/Cas-based Biosensor in Detection of Food-Borne Pathogens
10.19756/j.issn.0253-3820.231359
- VernacularTitle:基于CRISPR/Cas的生物传感器在食源性病原菌检测中的应用进展
- Author:
Xiao-Yuan ZHANG
1
;
Zhi-Hao YAO
;
Kai-Yu HE
;
Hong-Mei WANG
;
Xia-Hong XU
;
Zu-Fang WU
;
Liu WANG
Author Information
1. 宁波大学食品与药学学院,宁波 315000;浙江省农业科学院农产品质量安全与营养研究所,杭州 310021
- Keywords:
Food-borne pathogens;
CRISPR/Cas;
Biosensor;
Review
- From:
Chinese Journal of Analytical Chemistry
2024;52(4):469-480
- CountryChina
- Language:Chinese
-
Abstract:
Rapid and accurate detection methods for food-borne pathogens are essential to ensure food safety and human health.One promising innovation in this area is the clustered regularly interspaced short palindromic repeats/CRISPR-associated systems(CRISPR/Cas)biosensor,which utilizes Cas protein and CRISPR RNA(crRNA)ribonucleo protein to specifically recognize target genes,and converts target signals into detectable physical and chemical signals.The CRISPR/Cas biosensor shows many advantages,such as high specificity,programmability,and ease of use,making it promising to pathogen detection.This paper introduced the principles and characteristics of CRISPR/Cas systems,along with the strategies for signal recognition,amplification,and output based on different CRISPR/Cas biosensors,and their respective applications in food-borne pathogen detection.Furthermore,the construction principles and challenges of multiple biosensors based on CRISPR/Cas were explored,as well as their potential for simultaneous detection of multiple pathogens.Finally,the challenges and future development trends of CRISPR/Cas-based biosensors in rapid pathogen detection were discussed,aiming to provide valuable reference and inspiration for biosensor designers and food safety practitioners.
