Differentiation of bone marrow mesenchymal stem cells into cardiomyocytes with FGF-2 and Panax notoginseng saponins
10.16016/j.2097-0927.202404018
- VernacularTitle:FGF-2和三七总皂苷诱导骨髓间充质干细胞向心肌样细胞分化
- Author:
Zihan DONG
1
;
Haiping WANG
;
Yang LYU
;
Yang LIU
Author Information
1. 075000 河北张家口,河北北方学院基础医学院组织学与胚胎学教研室;075000 河北张家口,河北北方学院干细胞与生殖生物学实验室
- Keywords:
basic fibroblast growth factor;
Panax notoginseng saponins;
bone marrow mesenchymal stem cells;
cardiomyocytes;
myocardial infarction;
PI3K/Akt pathway
- From:
Journal of Army Medical University
2024;46(21):2415-2423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of basic fibro-blast growth factor(FGF-2)and Panax notoginseng saponins(PNS)alone or combined together on the differentiation of bone marrow mesenchymal stem cells(BMSCs)into cardiomyocytes(CMs)and the mechanism of action of PNS and FGF-2 in the differentiation.Methods BMSCs were isolated from 3-week-old SD rats(20～30 g)by whole bone marrow adherence,and then the purity of BMSCs was identified by flow cytometry.After the purity was qualified,PNS and FGF-2 alone or in combination,or combined with LY294002(phosphatidylinositol 3 kinase inhibitor),were used to induce BMSCs to differentiate.According to the corresponding culture time,the cells were divided into control group,PNS group,FGF-2 group,and PNS+FGF-2 group.The expression levels of Desmin and Cx43 after induction were detected by immunohistochemical assay.Western blotting was used to measure the expression levels of myocardium-specific proteins,related pathway proteins,and apoptosis-related proteins.RT-qPCR was conducted to detect the expression of myocardial transcription factors.Results The expression levels of myocardium-specific proteins and genes were increased in each single induction group(PNS group and FGF-2 group)and combined induction group(PNS+FGF-2 group,P＜0.05),and the highest expression levels were observed in the combined group.PNS and FGF-2 combined with LY294002 decreased the expression levels of myocardium-specific proteins and genes,decreased the phosphorylation of pathway-related protein Akt,decreased the level of Bcl-2,increased the level of Bax,and decreased the ratio of Bcl-2/Bax when compared with the 2 single induction groups(P＜0.05).Conclusion Both PNS and FGF-2 can induce BMSCs to differentiate into CMs after induction,and the combined induction is more effective in the differentiation.The PI3K/Akt signaling pathway is involved in the differentiation process by PNS and FGF-2,and PNS and FGF-2 play a role in reducing apoptosis.
