Bidirectional Mendelian randomization study on association between interleukin-9 level change and asthma
10.3969/j.issn.1671-8348.2024.18.018
- VernacularTitle:白细胞介素-9水平变化与哮喘关联的双向孟德尔随机化研究
- Author:
Xiujuan LI
1
;
Wenqin ZHAO
Author Information
1. 重庆医科大学附属璧山医院/重庆市璧山区人民医院儿科,重庆 402760
- Keywords:
interleukin-9;
asthma;
Mendelian randomization;
single nucleotide polymorphism locus
- From:
Chongqing Medicine
2024;53(18):2815-2819
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between asthma and IL-9 level,to provide reference for early prevention of asthma occurrence and improvement of asthma condition.Methods The data of single nucleotide polymorphism (SNP) were collected from two genome-wide association studies (GWAS) related to asthma and IL-9.Forty-three SNP instrumental variables associated with IL-9 were screened to conduct the forward direction Mendelian randomization (MR) analysis with asthma as the outcome.The three SNP associ-ated with asthma served as the instrumental variables,the IL-9 level change served as the study outcome to conduct the reverse MR analysis.Heterogeneity was detected by using Cochran's Q test,and the pleiotropy was detected by using the MR-Egger regression method.The robustness of the results was tested by the a leave-one-out method.Results The Q-test showed that no heterogeneity existed (P=0.383,0.883),and the MR analysis of fixed-effects model was used.The IL-9 level increase led to that the asthma occurrence risk was increased by 17％ (OR=1.17,95％CI:1.02-1.34).The asthma occurrence led to the IL-9 level increase by 0.09 standard deviation (OR=1.09,95％CI:1.01-1.19).After removing SNP one by one,the MR analy-sis results were steady.The MR-Egger regression method did not find the pleiotropy of instrumental variables (P=0.995,0.129).Conclusion There are bidirectional gene association between the IL-9 level change and asthma occurrence risk.
