Genetic analysis of wild-type hepatitis A virus strains
- Keywords: genetic analysis; hepatitis A virus; geographical region甲型肝炎病毒野毒株基因分型的研究陈勇 毛江森 洪艳 杨连华 凌志强 俞为群
- From: Chinese Medical Journal 2001;114(4):422-423
- CountryChina
- Language:Chinese
- Abstract: Objective To clarify the distribution of hepatitis A virus (HAV)genotype in geographical regions of China. Methods Seventeen representative HAV strains were isolated from the stool or serum of hepatitis A patients in different geographical regions. Viral RNA was recovered from stool or serum by proteinase K digestion and phenol-chloroform extraction, followed by ethanol precipitation prior to reverse transcription and polymerase chain reaction (RT-PCR) amplification. The nucleotide sequences of VP1/2A junction region were tested by using a direct sequencing technique. Results A pairwise comparison of sequences within 168 bases at the VP1/2A junction revealed that all the sequences clustered within genotype Ⅰ. About 53% of strains clustered in genotype ⅠB, with less than 6% variability; while the others clustered in genotype ⅠA, with less than 5.3% variability. Sequence homology between genotype ⅠA and ⅠB varied from 88.7% to 92.3%. Conclusion Epidemic or sporadic HAV strains in China may belong to HAV genotype ⅠA or ⅠB. Epidemiologically related strains may be identical or closely related in sequence.