Simian virus 40 large tumor antigen forms specific complexes with p53 and pRb in human brain tumors
- Keywords: simian virus 40; large tumor antigen (Tag); brain tumor; p53; pRb; tumor suppressor人脑肿瘤中SV40大T抗原与p53、pRb形成特异性复合物甄海宁 章翔 张志文 费舟 贺晓生 梁景文 刘先珍 黄文晋 张萍
- From: Chinese Medical Journal 2001;114(4):382-386
- CountryChina
- Language:Chinese
- Abstract: Objective To study the role of simian virus 40 (SV40) earlyregion gene coding product large tumor antigen (Tag) expression and the interaction between Tag and tumor suppressors p53 and pRb in human brain tumorigenesis. Methods Tag was investigated by immunoprecipitation followed by silver staining and Western blot in 65 cases of human brain tumors and 8 cases of normal brain tissues. Tag-p53 and Tag-pRb complexes were screened in 18 and 15 Tag positive tumor tissues, respectively. Results Tag was found in all 8 ependymomas and 2 choroid plexus papillomas, 90% of pituitary adenomas (9/10), 73% of astrocytomas (11/15), 70% of meningiomas (7/10), 50% of glioblastomas multiforme (4/8), 33% of medulloblastomas (2/6). 5 oligodendrogliomas, 1 pineocytoma, and 8 normal brain tissues were negative for Tag. Tag-p53 complex was detected in all 18 Tag positive tumors. Tag-pRb complex was found in all 15 Tag positive tumors. Conclusion SV40 Tag is expressed in human brain tumors and can form specific complexes with tumor suppressors p53 and pRb. The inactivation of p53 and pRb due to the formation of Tag-p53 and Tag-pRb complexes may be an important mechanism in the etiopathogenesis of human brain tumors.