Analysis of hypermutation of the 5' noncoding region in the BCL-6 gene
10.3760/j.issn:0366-6999.2001.03.013
- VernacularTitle:BCL-6基因5'非编码区基因突变的分析
- Keywords:
BCL-6 gene 5' noncoding region hypermutation diffuse large-cell lymphoma follicular lymphoma
- From:
Chinese Medical Journal
2001;114(3):286-290
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate BCL-6 gene mutations in Chinese populations with B-cell non-Hodgkin's lymphoma. Methods Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct DNA sequencing were used to identify mutations in the 5' noncoding region of the BCL-6 gene in a total of 40 cases of diffuse large-cell lymphoma (DLCL) and follicular lymphoma (FL). Results Nine cases were found to have base substitutions. The incidence of BCL-6 gene mutation and the frequency of single-base changes were approximately 25.7% and (0.56-1.10)×10-2/bp, respectively. Conclusions The 5' regulatory region of the BCL-6 gene undergoes frequent somatic hypermutation during lymphomagenesis and the identification of BCL-6 gene hypermutations provides a molecular marker for confirmatory diagnosis of B-NHL.
