Expression of transcription inhibitor zinc finger protein 57 in breast cancer and its clinical significance
10.7619/jcmp.201723016
- VernacularTitle:转录抑制因子锌指蛋白57在乳腺癌中的表达及意义
- Author:
Mingjie ZHENG
1
;
Shui WANG
;
Lie CHEN
;
Ge MA
Author Information
1. 南京医科大学附属第一医院乳腺外科
- Keywords:
Zinc finger protein 57;
breast cancer;
lymph node involvement
- From:
Journal of Clinical Medicine in Practice
2017;21(23):53-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of zinc finger protein 57 (ZFP57) in breast cancer and its clinical significance.Methods The ZFP57 expressions were detected by quantitative real-time PCR(QPCR) and Western Blot technique.And its expressions in breast cancer and para-tumorous tissues in 80 patients were detected by QPCR method,and its correlation with clinical and pathogenic results were explored.Results In these breast cancer cells (MDA-MB-231,SUM1315,T47D,MCF-7 and ZR-75-1),the expression contents of ZFP57 in mRNA level detected by QPCR was (2.866 ± 0.1986),(1.000 ± 0.09928),(6.362 ± 0.2970),(10.15 ± 0.4218),(7.800 ± 0.6797),respectively,(P ＜ 0.05),while in protein level was (2.689 ± 0.09051),(1.018 ± 0.05613),(7.044 ± 0.1232),(9.328 ± 0.2981),(7.390 ± 0.2332),respectively,and the differences were statistically significant (P ＜ 0.05).The average expression of ZFP57 in breast carcinoma was (0.0558 ± 0.0101),while was (0.1708 ± 0.0267) in para-carcinoma and they showed significant differences (P ＜ 0.05).The clinical pathogenic analysis revealed that there were significant differences in the average expression of ZFP57 in breast carcinoma without lymph nodes involvement and with lymph nodes involvement [(0.0707 ± 0.0138) vs.(0.0209 ± 0.0064),P ＜ 0.05].The average expression of ZFP57 in masses smaller than 2 cm was (0.0957 ± 0.0215),was (0.0418 ± 0.0111) in masses between 2 ～ 5cm,and (0.0198 ± 0.0097) in masses larger than 5 cm,which showed significant differences (P ＜ 0.05).Conclusion The expression of ZFP57 in breast carcinoma is decreased.And the expression of ZFP57 is negatively correlated with the lymph nodes involvement and the size of masses.
