Proliferation and invasion of ovarian cancer SK-OV-3 cells inhibited by Tectoridin
10.3760/cma.j.cn112152-20200206-00072
- VernacularTitle:射干苷对卵巢癌SK－OV－3细胞增殖迁移和侵袭的影响
- Author:
Lijuan WANG
1
;
Huirong SHI
Author Information
1. 驻马店市中心医院妇科 463000
- Keywords:
Ovarian neoplasms;
Tectoridin;
Migration;
PI3K/AKT
- From:
Chinese Journal of Oncology
2020;42(7):570-574
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Tectoridin on the proliferation, migration and invasion of ovarian cancer SK-OV-3 cells and its mechanism.Methods:SK-OV-3 cells were treated with 0, 5, 10, 50 and 100 μg/L Tectoridin for 24 hours. The proliferation of SK-OV-3 cells treated with different concentrations of Tectoridin was detected by cell counting kit-8 (CCK-8). The migration was analyzed by wound healing test and the invasion was observed by Transwell. The effects of different concentrations of Tectoridin on the protein levels of phosphoinositide 3-kinase (PI3K)/serine-threonine kinase (AKT) signaling pathway were detected by western blot assay.Results:The A values of 0, 5, 10, 50, 100 μg/L Tectoridin groups were 0.857±0.043, 0.725±0.036, 0.611±0.032, 0.410±0.027, and 0.321±0.023, respectively. The relative migration distances of the cells were 1.000±0.083, 0.896±0.092, 0.644±0.075, 0.432±0.056, and 0.215±0.043, respectively. The numbers of cell invasion were (106.258±11.785), (93.241±10.251), (74.258±7.963), (40.236±5.210), and (32.147±3.458), respectively. The phosphorylated PI3K (p-PI3K) protein levels were 1.000±0.079, 0.875±.089, 0.727±0.075, 0.452±0.053, 0.365±0.052, respectively. The phosphorylated AKT (p-AKT) protein levels were 1.000±0.070, 0.816±0.072, 0.635±0.079, 0.463±0.065, 0.305±0.047, respectively. Compared with the 0 μg/L Tectoridin group, the differences of 5, 10, 50, 100 μg/L Tectoridin group were statistically significant (all P<0.05). The A values of the control group and the LY294002 group were 0.873±0.081 and 0.494±0.038, respectively. The relative cell migration distances were 1.000±0.081 and 0.523±0.051, respectively. The numbers of cell invasion were (106.228±11.783) and (61.243±9.251), respectively. The protein levels of p-PI3K were 1.000±0.075, 0.521±0.046, respectively. The protein levels of p-AKT were 1.000±0.070, 0.465±0.051, respectively. Compared with the control group, the difference in the LY294002 group was statistically significant ( P<0.05). Conclusion:Tectoridin may inhibit proliferation, migration and invasion of SK-OV-3 cells by regulating PI3K/AKT signaling pathway.
