Determination of dimethyl oxalate and diethyl oxalate in workplace air by high performance liquid chromatography
10.3760/cma.j.cn121094-20230209-00039
- VernacularTitle:工作场所空气中草酸二甲酯和草酸二乙酯同时测定的高效液相色谱法
- Author:
Zhiliang CHEN
1
;
Min WANG
;
Jinfeng JIANG
;
Weili SONG
Author Information
1. 武汉市职业病防治院卫生检验科，武汉 430015
- Keywords:
Workplace;
Dimethyl oxalate;
Diethyl oxalate;
High performance liquid;
Chromatography;
Air
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2024;42(3):199-202
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a high performance liquid chromatography method for the simultaneous determination of dimethyl oxalate (DMO) and diethyl oxalate (DEO) in workplace air.Methods:From January 2022 to January 2023, air samples were collected by silica gel tubes, desorbed by acetonitrile, separated by C18 chromatographic column, detected by photo-array detector, and retention time was used to characterize and peak area was used to quantify at 210 nm wavelength.Results:The linear relationships of DMO and DEO were good, r>0.999. The detection limits of DMO and DEO were 0.39 and 0.52 μg/ml, respectively. The quantitative limit was 1.28 μg/ml for DMO and 1.72 μg/ml for DEO. Average desorption efficiency for DMO was 82.40%-92.72%, and DEO was 94.13%-97.69%. The intra-assay precision of DMO was 1.87%-6.18%, and DEO was 2.25%-3.31%. Inter-assay precision of DMO was 3.29%-5.73%, and DEO was 1.38%-2.94%. Average sampling efficiencies were 100% for both DMO and DEO. Breakthrough capacity of DMO was 37.61 mg (200 mg solid adsorbent), DEO was >28.11 mg (200 mg solid adsorbent). Samples should be stored at 4 ℃ for at least 7 days. Conclusion:This method is easy to operate and has strong practicability. All indicators meet the requirements of the specification, and it is suitable for the simultaneous determination of DMO and DEO in the workplace air.
