Expression and functional assessment of solute carrier 26A transporter family in HEK-293 cells
- VernacularTitle:溶质转运体26A家族在HEK-293细胞中的表达和功能
- Author:
Chao FAN
1
;
Mingyu FU
;
Zhongju XIAO
;
Jie TANG
Author Information
1. 南方医科大学基础医学院生理学教研室
- Keywords:
human embryonic kidney 293 cells;
solute carrier protein 26A;
whole-cell patch recording;
nonlinear capacitance
- From:
Journal of Southern Medical University
2015;(6):801-806,811
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express solute carrier 26A proteins in HEK-293 cells and explore their functions. Methods SLC26A-eGFP plasmids were transiently transfected into HEK-293 cells, and the nonlinear capacitance of the cells expressing SLC26A proteins was measured by whole-cell patch recording. Results All the SLC26A transporters were expressed on the membrane of HEK-293 cells. Each member of the SLC26A transporter family showed robust nonlinear capacitance, which represented their binding capability with anions. Conclusion The SLC26A transporters expressed on HEK cells show similar functions as expected in tissue environment. The plasmids we constructed facilitate structural and functional study of SLC26A transporters.
